SIRT1 Antikörper (AA 236-490)

Produktnummer ABIN1078528

Produktdetails anti-SIRT1 Antikörper

Target: SIRT1 (Sirtuin 1 (SIRT1))

Bindungsspezifität: AA 236-490

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck: Polyclonal Antibody to Sirtuin 1 (SIRT1)

Spezifität: The antibody is a rabbit polyclonal antibody raised against SIRT1. It has been selected for its ability to recognize SIRT1 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Sirtuin 1 (SIRT1) corresdonding to Glu236~Ala490 with N-terminal His Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: Sirtuin 1 (SIRT1 Produkte)

Synonyme: sirtuin 1 antikoerper, AA673258 antikoerper, SIR2L1 antikoerper, Sir2 antikoerper, Sir2a antikoerper, Sir2alpha antikoerper, sir2l1 antikoerper, sirtuin antikoerper, SIR2 antikoerper, sirtuin 1 antikoerper, sirtuin 1 L homeolog antikoerper, SIRT1 antikoerper, SRT1 antikoerper, Sirt1 antikoerper, sirt1.L antikoerper

Hintergrund: SIR2L1, Silent Mating Type Information Regulation 2 Homolog 1, NAD-Dependent Deacetylase Sirtuin-1, SIR2-like protein 1, SirtT1 75 kDa fragment

Pathways: MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling