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WNT9B Antikörper (Internal Region)

WNT9B Reaktivität: Human IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049490
  • Target Alle WNT9B Antikörper anzeigen
    WNT9B (Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B))
    Bindungsspezifität
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 24
    • 8
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 22
    • 1
    • 1
    Kaninchen
    Klonalität
    • 23
    • 1
    Polyklonal
    Konjugat
    • 14
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser WNT9B Antikörper ist unkonjugiert
    Applikation
    • 11
    • 9
    • 4
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human WNT9B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Monkey, Panda, Bat, Bovine, Horse, Pig (93%) Mouse, Hamster, Dog (87%) Rat, Rabbit (80%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 15 amino acid peptide from internal region of human WNT9B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%), Monkey, Panda, Bat, Bovine, Horse, Pig (93%), Mouse, Hamster, Dog (87%), Rat, Rabbit (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product WNT9B Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (10 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    WNT9B (Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B))
    Andere Bezeichnung
    WNT9B / WNT15 (WNT9B Produkte)
    Synonyme
    WNT14B antikoerper, WNT15 antikoerper, WNT9B antikoerper, wnt-9b antikoerper, Wnt14b antikoerper, Wnt15 antikoerper, clf antikoerper, clf1 antikoerper, wnt-14b antikoerper, wnt-15 antikoerper, Wnt family member 9B antikoerper, wingless-type MMTV integration site family member 9B L homeolog antikoerper, protein Wnt-9b antikoerper, wingless-type MMTV integration site family, member 9B antikoerper, WNT9B antikoerper, wnt9b.2.L antikoerper, Wnt9b antikoerper, LOC468296 antikoerper, wnt9b antikoerper
    Hintergrund
    Name/Gene ID: WNT9B
    Family: WNT

    Synonyms: WNT9B, WNT14B, WNT15, Protein Wnt-14b, Protein Wnt-15, Protein Wnt-9b
    Gen-ID
    7484
    Pathways
    WNT Signalweg, Tube Formation
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