Tetraspanin 6 Antikörper (Internal Region)

Produktnummer ABIN1049447

Produktdetails anti-Tetraspanin 6 Antikörper

Target: Tetraspanin 6 (TSPAN6)

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tetraspanin 6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human TSPAN6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%) Gibbon, Bovine, Horse (94%) Monkey, Galago, Marmoset, Bat, Dog, Pig (89%) Mouse, Rat, Hamster, Guinea pig (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from internal region of human TSPAN6. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%), Gibbon, Bovine, Horse (94%), Monkey, Galago, Marmoset, Bat, Dog, Pig (89%), Mouse, Rat, Hamster, Guinea pig (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Tetraspanin 6

Target: Tetraspanin 6 (TSPAN6)

Andere Bezeichnung: TM4SF6 / TSPAN6 (TSPAN6 Produkte)

Synonyme: GB14865 antikoerper, TSPAN6 antikoerper, t245 antikoerper, tm4sf6 antikoerper, tspan-6 antikoerper, DKFZp469J2433 antikoerper, TM4SF6 antikoerper, T245 antikoerper, TSPAN-6 antikoerper, 6720473L21Rik antikoerper, AI316786 antikoerper, Tm4sf antikoerper, Tm4sf6 antikoerper, tetraspanin 6 antikoerper, TSPAN6 antikoerper, Tspan6 antikoerper, tspan6 antikoerper

Hintergrund: Name/Gene ID: TSPAN6
Family: Tetraspan

Synonyms: TSPAN6, A15 homolog, Tetraspanin TM4-D, Tetraspanin-6, TM4SF6, Tetraspanin 6, T245, T245 protein, Tetraspan TM4SF, TSPAN-6

Gen-ID: 7105