TAAR1 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1049377

Produktdetails anti-TAAR1 Antikörper

Target: TAAR1 (Trace Amine Associated Receptor 1 (TAAR1))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TAAR1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marke: IHC-plus™

Spezifität: Human TAAR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human (100%) Chimpanzee (95%) Gorilla, Orangutan, Monkey (89%) Gibbon (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human TAAR1. Percent identity with other species by BLAST analysis: Human (100%), Chimpanzee (95%), Gorilla, Orangutan, Monkey (89%), Gibbon (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (8 - 19 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu TAAR1

Target: TAAR1 (Trace Amine Associated Receptor 1 (TAAR1))

Andere Bezeichnung: TAAR1 / TA1 (TAAR1 Produkte)

Synonyme: RP11-295F4.9 antikoerper, TA1 antikoerper, TAR1 antikoerper, TRAR1 antikoerper, Tar1 antikoerper, Trar1 antikoerper, taR-1 antikoerper, si:dkey-7c18.14 antikoerper, trace amine associated receptor 1 antikoerper, trace amine-associated receptor 1 antikoerper, trace-amine-associated receptor 1 antikoerper, trace amine associated receptor 1b antikoerper, TAAR1 antikoerper, Taar1 antikoerper, taar1b antikoerper

Hintergrund: Name/Gene ID: TAAR1
Subfamily: Trace amine
Family: GPCR

Synonyms: TAAR1, RP11-295F4.9, RTAR1, Snorf33, TRAR1, TaR-1, TAR1, TA1, Trace amine receptor 1

Gen-ID: 134864