SLC4A2 Antikörper (N-Term)

Produktnummer ABIN1049340

Produktdetails anti-SLC4A2 Antikörper

Target: SLC4A2 (Solute Carrier Family 4, Anion Exchanger, Member 2 (erythrocyte Membrane Protein Band 3-Like 1) (SLC4A2))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Schwein, Kaninchen, Hund, Pferd, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC4A2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human SLC4A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Rabbit, Pig (100%) Monkey, Bovine, Guinea pig (94%) Elephant (89%) Bat, Opossum (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from N-Terminus of human SLC4A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Rabbit, Pig (100%), Monkey, Bovine, Guinea pig (94%), Elephant (89%), Bat, Opossum (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu SLC4A2

Target: SLC4A2 (Solute Carrier Family 4, Anion Exchanger, Member 2 (erythrocyte Membrane Protein Band 3-Like 1) (SLC4A2))

Andere Bezeichnung: SLC4A2 / AE2 (SLC4A2 Produkte)

Synonyme: AE2 antikoerper, BND3L antikoerper, EPB3L1 antikoerper, HKB3 antikoerper, NBND3 antikoerper, SLC4A2 antikoerper, B3RP antikoerper, Ae2 antikoerper, Aep2 antikoerper, AE 2 antikoerper, Anion exchanger 2 antikoerper, solute carrier family 4 member 2 antikoerper, anion exchange protein 2 antikoerper, solute carrier family 4 (anion exchanger), member 2 antikoerper, SLC4A2 antikoerper, LOC100599599 antikoerper, Slc4a2 antikoerper

Hintergrund: Name/Gene ID: SLC4A2
Subfamily: Anion exchanger
Family: Transporter

Synonyms: SLC4A2, Anion exchange protein 2, AE 2, Anion exchanger 2, Anion exchanger 2 type a, Anion exchanger 2 type b1, Anion exchanger 2 type b2, BND3L, EPB3L1, AE2, HKB3, MPB3L, Anion exchanger type 2, Erythrocyte band 3, NBND3

Gen-ID: 6522