SCN3A Antikörper (Internal Region)

Produktnummer ABIN1049321

Produktdetails anti-SCN3A Antikörper

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SCN3A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human SCN3A / Nav1.3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee (100%) Monkey, Galago (94%) Orangutan, Gibbon, Marmoset, Mouse, Bovine, Guinea pig (89%) Rat, Hamster, Panda, Dog (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from internal region of human SCN3A / Nav1.3. Percent identity with other species by BLAST analysis: Human, Chimpanzee (100%), Monkey, Galago (94%), Orangutan, Gibbon, Marmoset, Mouse, Bovine, Guinea pig (89%), Rat, Hamster, Panda, Dog (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (15 - 20 μg/mL)

Usage: Proteinase K antigen retrieval was used for Immunohistochemistry with formalin-fixed paraffin-embedded human tissues.

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu SCN3A

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Andere Bezeichnung: SCN3A / Nav1.3 (SCN3A Produkte)

Synonyme: SCN3A antikoerper, nav1.3 antikoerper, Gm1000 antikoerper, Nav1.3 antikoerper, SCIII antikoerper, Scn2a antikoerper, NAC3 antikoerper, sodium voltage-gated channel alpha subunit 3 antikoerper, sodium channel, voltage gated, type III alpha subunit antikoerper, sodium channel protein type 9 subunit alpha antikoerper, sodium channel, voltage-gated, type III, alpha antikoerper, sodium channel protein type 3 subunit alpha antikoerper, SCN3A antikoerper, scn3a antikoerper, LOC100180733 antikoerper, Scn3a antikoerper, LOC534223 antikoerper

Hintergrund: Name/Gene ID: SCN3A
Subfamily: Sodium channel - voltage-gated
Family: Ion Channel

Synonyms: SCN3A, NAC3, Nav1.3, SCIII, Brain sodium channel III, KIAA1356

Gen-ID: 6328