Prokineticin Receptor 1 Antikörper (N-Term)

Produktnummer ABIN1049243

Produktdetails anti-Prokineticin Receptor 1 Antikörper

Target: Prokineticin Receptor 1 (PROKR1)

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prokineticin Receptor 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human PROKR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset (93%) Elephant, Bovine (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from N-terminal extracellular domain of human PROKR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (93%), Elephant, Bovine (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (7 - 16 μg/mL), WB

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Prokineticin Receptor 1

Target: Prokineticin Receptor 1 (PROKR1)

Andere Bezeichnung: PROKR1 (PROKR1 Produkte)

Synonyme: EG-VEGFR1 antikoerper, Gpr73 antikoerper, Pkr1 antikoerper, GPR73 antikoerper, GPR73a antikoerper, PKR1 antikoerper, ZAQ antikoerper, PROKR1 antikoerper, prokineticin receptor 1 antikoerper, Prokr1 antikoerper, PROKR1 antikoerper

Hintergrund: Name/Gene ID: PROKR1
Subfamily: Prokineticin
Family: GPCR

Synonyms: PROKR1, G protein-coupled receptor 73, GPCR73A, G protein-coupled receptor ZAQ, G-protein coupled receptor 73, G-protein coupled receptor ZAQ, GPR73, GPR73a, PK-R1, Prokineticin receptor 1, EG-VEGF receptor, PKR1, ZAQ

Gen-ID: 10887

Pathways: Hedgehog Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma