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NR5A2 + LRH1 Antikörper (C-Term)

NR5A2 Reaktivität: Human, Rind (Kuh), Schaf, Hund, Pferd, Schwein, Affe IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049146
  • Target Alle NR5A2 + LRH1 (NR5A2) Antikörper anzeigen
    NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))
    Bindungsspezifität
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 46
    • 22
    • 19
    • 7
    • 7
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Human, Rind (Kuh), Schaf, Hund, Pferd, Schwein, Affe
    Wirt
    • 43
    • 5
    • 2
    Kaninchen
    Klonalität
    • 45
    • 5
    Polyklonal
    Konjugat
    • 34
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser NR5A2 + LRH1 Antikörper ist unkonjugiert
    Applikation
    • 35
    • 17
    • 12
    • 6
    • 3
    • 2
    • 2
    • 2
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Dog, Elephant, Panda, Horse, Pig (100%) Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback (94%) Mouse, Rat, Trout, Pufferfish, Zebrafish (88%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from C-terminal to ligand-binding domain of human NR5A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Dog, Elephant, Panda, Horse, Pig (100%), Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback (94%), Mouse, Rat, Trout, Pufferfish, Zebrafish (88%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product NR5A2 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (7 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))
    Andere Bezeichnung
    NR5A2 / LRH-1 (NR5A2 Produkte)
    Synonyme
    xff1rashort antikoerper, NR5A2 antikoerper, b1f antikoerper, b1f2 antikoerper, cpf antikoerper, ff1r antikoerper, ftf antikoerper, ftz-f1 antikoerper, ftz-f1beta antikoerper, hb1f antikoerper, hb1f-2 antikoerper, lrh-1 antikoerper, lrh1 antikoerper, xff1ra antikoerper, B1F antikoerper, B1F2 antikoerper, CPF antikoerper, FTF antikoerper, FTZ-F1 antikoerper, FTZ-F1beta antikoerper, LRH-1 antikoerper, LRH1 antikoerper, hB1F-2 antikoerper, xFF1rB antikoerper, AU020803 antikoerper, D1Ertd308e antikoerper, Ftf antikoerper, UF2-H3B antikoerper, Lrh-1 antikoerper, nuclear receptor subfamily 5 group A member 2 S homeolog antikoerper, nuclear receptor subfamily 5 group A member 2 antikoerper, nuclear receptor subfamily 5 group A member 2 L homeolog antikoerper, nuclear receptor subfamily 5, group A, member 2 antikoerper, nr5a2.S antikoerper, NR5A2 antikoerper, nr5a2 antikoerper, nr5a2.L antikoerper, Nr5a2 antikoerper
    Hintergrund
    Name/Gene ID: NR5A2
    Subfamily: NR5 Fushi tarazu-like
    Family: NHR

    Synonyms: NR5A2, B1F, B1F2, B1-binding factor, CPF, CYP7A promoter-binding factor, FTZ-F1beta, FTF, Liver receptor homolog 1, LRH1, LRH-1, HB1F, HB1F-2, Nuclear receptor phr-1, FTZ-F1, Liver receptor homolog-1, Nuclear receptor NR5A2, Receptor phr
    Gen-ID
    2494
    Pathways
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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