NR2E1 Antikörper (N-Term)

Produktnummer ABIN1049135

Produktdetails anti-NR2E1 Antikörper

Target: NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))

Bindungsspezifität: N-Term

Reaktivität: Human, Kaninchen, Rind (Kuh), Hund, Pferd, Schwein, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NR2E1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human NR2E1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Shrew, Bat, Bovine, Dog, Elephant, Panda, Rabbit, Horse, Pig, Opossum, Armadillo (100%) Mouse, Rat, Hamster, Sparrow, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish, Stickleback (93%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from a N-terminal region of human NR2E1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Shrew, Bat, Bovine, Dog, Elephant, Panda, Rabbit, Horse, Pig, Opossum, Armadillo (100%), Mouse, Rat, Hamster, Sparrow, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish, Stickleback (93%).

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5.5 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu NR2E1

Target: NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))

Andere Bezeichnung: NR2E1 / TLX (NR2E1 Produkte)

Synonyme: NR2E1 antikoerper, TLL antikoerper, TLX antikoerper, XTLL antikoerper, Mtl1 antikoerper, Mtll antikoerper, Tlx antikoerper, fierce antikoerper, frc antikoerper, tailless antikoerper, fc30e03 antikoerper, fc39f12 antikoerper, wu:fc30e03 antikoerper, wu:fc39f12 antikoerper, zgc:100991 antikoerper, Xtll antikoerper, nr2e1-A antikoerper, tlx antikoerper, nuclear receptor subfamily 2 group E member 1 antikoerper, nuclear receptor subfamily 2, group E, member 1 antikoerper, nuclear receptor subfamily 2 group E member 1 S homeolog antikoerper, NR2E1 antikoerper, Nr2e1 antikoerper, nr2e1 antikoerper, nr2e1.S antikoerper

Hintergrund: Name/Gene ID: NR2E1
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR

Synonyms: NR2E1, HTll, Tailless homolog, Tailless, TLX, TLL, Nuclear receptor TLX, Protein tailless homolog, Tailes-related receptor, XTLL

Gen-ID: 7101

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance