KCNN4 Antikörper (C-Term)

Produktnummer ABIN1048993

Produktdetails anti-KCNN4 Antikörper

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein, Hund, Kaninchen, Meerschweinchen, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KCNN4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human KCNN4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Rabbit, Pig, Guinea pig (100%) Monkey, Horse, Platypus (93%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 14 amino acid peptide from C-Terminus of human KCNN4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Rabbit, Pig, Guinea pig (100%), Monkey, Horse, Platypus (93%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu KCNN4

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Andere Bezeichnung: KCNN4 / KCa3.1 (KCNN4 Produkte)

Synonyme: KCa3.1 antikoerper, IK1 antikoerper, IKCA1 antikoerper, KCA4 antikoerper, SK4 antikoerper, hIKCa1 antikoerper, hKCa4 antikoerper, hSK4 antikoerper, rKCNN4c antikoerper, rSK4 antikoerper, SKCas antikoerper, mIKCa1 antikoerper, potassium calcium-activated channel subfamily N member 4 antikoerper, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 antikoerper, KCNN4 antikoerper, Kcnn4 antikoerper

Hintergrund: Name/Gene ID: KCNN4
Subfamily: Potassium channel - calcium-activated
Family: Ion Channel

Synonyms: KCNN4, HIKCa1, HKCa4, HIK1, IK1, KCA4, HSK4, IKCA1, KCa3.1, SK4, SKCa 4, Putative Gardos channel, SKCa4

Gen-ID: 3783