PTGER2 Antikörper (Extracellular Domain)

Produktnummer ABIN1048569

Produktdetails anti-PTGER2 Antikörper

Target: PTGER2 (Prostaglandin E Receptor 2 (Subtype EP2), 53kDa (PTGER2))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTGER2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human EP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%) Elephant, Bovine, Dog (94%) Rat, Panda, Pig (88%) Mouse (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd extracellular domain of human EP2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%), Elephant, Bovine, Dog (94%), Rat, Panda, Pig (88%), Mouse (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (6 - 15 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PTGER2

Target: PTGER2 (Prostaglandin E Receptor 2 (Subtype EP2), 53kDa (PTGER2))

Andere Bezeichnung: PTGER2 / EP2 (PTGER2 Produkte)

Synonyme: EP2 antikoerper, Ptgerep2 antikoerper, ptger2 antikoerper, ptger2l antikoerper, zgc:63974 antikoerper, MGC69060 antikoerper, PTGER2 antikoerper, Ptger-ep2 antikoerper, si:ch211-13n20.1 antikoerper, si:dkey-29m11.3 antikoerper, zgc:136608 antikoerper, prostaglandin E receptor 2 antikoerper, prostaglandin E receptor 2 (subtype EP2) antikoerper, prostaglandin E receptor 2a (subtype EP2) antikoerper, prostaglandin E receptor 2 S homeolog antikoerper, prostaglandin E receptor 2b (subtype EP2) antikoerper, PTGER2 antikoerper, Ptger2 antikoerper, ptger2a antikoerper, ptger2.S antikoerper, ptger2b antikoerper

Hintergrund: Name/Gene ID: PTGER2
Subfamily: Prostanoid
Family: GPCR

Synonyms: PTGER2, EP2 prostaglandin e2 receptor, EP2 prostaglandin receptor, EP2, Pge2-r ep2, PGE2 receptor EP2 subtype, Prostaglandin E receptor 2, Prostanoid EP2 receptor, EP2 receptor, PGE receptor EP2 subtype

Gen-ID: 5732