ACVR1C/ALK7 Antikörper (Internal Region)

Produktnummer ABIN1048286

Produktdetails anti-ACVR1C/ALK7 Antikörper

Target: ACVR1C/ALK7 (ACVR1C) (Activin Receptor Type 1C (ACVR1C))

Bindungsspezifität: Internal Region

Reaktivität: Human, Rind (Kuh), Schwein, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACVR1C/ALK7 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ACVR1C. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Bat, Pig (100%) Marmoset, Elephant, Panda, Horse, Rabbit (94%) Opossum, Turkey, Chicken, Platypus (88%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from internal region of human ACVR1C. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Bat, Pig (100%), Marmoset, Elephant, Panda, Horse, Rabbit (94%), Opossum, Turkey, Chicken, Platypus (88%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K.

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ACVR1C/ALK7

Target: ACVR1C/ALK7 (ACVR1C) (Activin Receptor Type 1C (ACVR1C))

Andere Bezeichnung: ACVR1C / ALK7 (ACVR1C Produkte)

Synonyme: ACVRLK7 antikoerper, ALK7 antikoerper, Alk-7 antikoerper, C230097P10 antikoerper, Alk7 antikoerper, habrec1 antikoerper, activin A receptor type 1C antikoerper, activin A receptor, type IC antikoerper, ACVR1C antikoerper, Acvr1c antikoerper

Hintergrund: Name/Gene ID: ACVR1C
Subfamily: Activin/TGFb
Family: Protein Kinase

Synonyms: ACVR1C, Activin receptor-like kinase 7, ACTR-IC, Activin receptor type IC, ACVRLK7, Activin receptor type-1C, ALK7, Activin A receptor, type IC, ALK-7

Gen-ID: 130399

Pathways: Negative Regulation of Hormone Secretion, Positive Regulation of Endopeptidase Activity