Ziege anti-Armenischer Hamster IgG (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN1044080

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Syrischer Goldhamster, Armenischer Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Golden Syrian and Armenian Hamster IgG and Golden Syrian and Armenian Hamster Serum.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Golden Syrian and Armenian Hamster IgG whole molecules

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:300-1:2000

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:5000-1:25,000

Western Blot Dilution: 1:2,000-1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to opening. This product is stable at 4 °C as an undiluted liquid. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -24 °C or below.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat F(ab')2 Anti-Hamster IgG Antibody Biotin Conjugation, Goat Fab2 Anti-Hamster IgG biotin Conjugated Antibody, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Biotin Conjugation, Goat Fab2 Anti-Golden Syrian & Armenian Hamster IgG biotin Conjugated Antibody

Background: F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Biotin Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.