Kaninchen anti-Maus IgG Antikörper (Atto 550)

Details zu Produkt Nr. ABIN1043989, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Maus
3172
3054
2511
1746
1580
820
766
504
483
430
411
409
335
291
277
173
159
128
54
30
30
26
15
12
12
8
7
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Kaninchen
7495
5380
2124
1082
552
357
89
73
55
35
23
15
8
5
1
1
1
Klonalität
Polyklonal
Konjugat
Atto 550
2298
1890
1829
1326
792
657
536
340
269
174
147
145
143
140
119
112
112
102
98
94
93
91
89
86
84
81
79
78
75
73
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
49
48
43
41
38
36
32
31
23
23
22
20
19
17
16
15
15
14
14
14
12
12
12
12
11
11
11
10
10
10
10
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen

Immunogen: highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Immunogen Type: Native Protein

Isotyp IgG
Produktmerkmale Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reinigung Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 550 was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Labeling Ratio 1.5
Antigen
Hintergrund

Synonyms: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO550 conjugation, rabbit anti-Mouse IgG ATTO550 conjugated Antibody

Background: Anti-Mouse IgG ATTO 550 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Applikations-hinweise

Application Note: Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Kommentare

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Lagerung RT,4 °C,-20 °C
Informationen zur Lagerung Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Rabbit anti-Mouse (Murine) IgG antibody (Atto 550) (ABIN1043989) Rabbit anti-Mouse (Murine) IgG antibody (Atto 550)
Western Blotting (WB) image for Rabbit anti-Mouse (Murine) IgG antibody (Atto 550) (ABIN1043989) Western Blot of ATTO 550 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibod...
 image for Rabbit anti-Mouse (Murine) IgG antibody (Atto 550) (ABIN1043989) Rabbit anti-Mouse (Murine) IgG antibody (Atto 550) (Image 3)