Kaninchen anti-Ziege IgG (Heavy & Light Chain) Antikörper (Atto 655) - Preadsorbed

Produktnummer ABIN1043949

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ziege

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Atto 655

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Spezifität: Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-AdsorbedAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.

Produktmerkmale: Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Goat IgG whole molecule

Isotyp: IgG

Labeling Ratio: 3.5

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Kommentare:

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: rabbit anti-Goat IgG ATTO 655 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO655 Conjugation

Background: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.