NYS48/HAUS8 Antikörper (Internal Region)

Produktnummer ABIN1043902

Produktdetails anti-NYS48/HAUS8 Antikörper

Target: NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NYS48/HAUS8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Anti-Hice1 Affinity purified antibody is directed against human Hice1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known.

Produktmerkmale: Hice1 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gammaTuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.

Sterilität: Sterile filtered

Immunogen: Anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Hice1 antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Kommentare:

Gene Name: Hice1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.45 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-NYS48/HAUS8 Antikörper (ABIN1043902)

Wu, Lin, Wei, Chen, Shan, Lee: "Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3652-62, (2008) (PubMed).

Details zu NYS48/HAUS8

Target: NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))

Andere Bezeichnung: HICE1 (HAUS8 Produkte)

Synonyme: 2410004L22Rik antikoerper, Hice1 antikoerper, NY-SAR-48 antikoerper, hice1 antikoerper, DGT4 antikoerper, HICE1 antikoerper, Ny-sar-48 antikoerper, RGD1311565 antikoerper, 4HAUS augmin-like complex, subunit 8 antikoerper, HAUS augmin like complex subunit 8 L homeolog antikoerper, HAUS augmin like complex subunit 8 antikoerper, HAUS augmin-like complex, subunit 8 antikoerper, Haus8 antikoerper, haus8.L antikoerper, HAUS8 antikoerper

Hintergrund: Hice1 is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the γTuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
Synonyms: HAUS8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48

Gen-ID: 93323

NCBI Accession: NP_219485

UniProt: Q9BT25