PSMA Antikörper

Produktnummer ABIN1042485

Produktdetails anti-PSMA Antikörper

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PSMA Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: 107.1A4 recognizes an antigen, which appeared to be distinct from those previously described and shows specific tumor targeting in preliminary in vivo studies. MAb 107-1A4 is suggested to recognize a distinct conformational and unique epitope in the extracellular domain of PSMA. PSMA is a type II membrane glycoprotein of 100kDa that has a short intracellular N-terminal domain (residues 1 to 18), a transmembrane region (residues 19 to 43) and a large extracellular domain consisting of residues 44 to 750.

Produktmerkmale: Membrane,Tumour marker

Aufreinigung: Supernatant

Klon: 107-1A4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: MAb 107-1A4 could not be used in Western blot, is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunocytochemistry, flow cytometry and ELISA. Optimal antibody dilution should be determined by titration, recommended range is 1:100 – 1:1000 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Kommentare:

107.1A4 is a mouse monoclonal IgG1/k antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from an immunized mouse, using a two-phase immunization protocol involving the prostate cancer cell line LNCaP.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.09% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-PSMA Antikörper (ABIN1042485)

Brown, Wegner, Wang, Buhler, Arfman, Lange, Vessella: "A novel monoclonal antibody 107-1A4 with high prostate specificity: generation, characterization of antigen expression, and targeting of human prostate cancer xenografts." in: Prostate cancer and prostatic diseases, Vol. 1, Issue 4, pp. 208-215, (2002) (PubMed).

Wang, Diamond, Hass, Sokoloff, Vessella: "Identification of prostate specific membrane antigen (PSMA) as the target of monoclonal antibody 107-1A4 by proteinchip; array, surface-enhanced laser desorption/ionization (SELDI) technology." in: International journal of cancer. Journal international du cancer, Vol. 92, Issue 6, pp. 871-6, (2001) (PubMed).

Details zu PSMA

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Andere Bezeichnung: PSMA (FOLH1 Produkte)

Synonyme: FGCP antikoerper, FOLH antikoerper, GCP2 antikoerper, GCPII antikoerper, NAALAD1 antikoerper, NAALAdase antikoerper, PSM antikoerper, PSMA antikoerper, mGCP antikoerper, mopsm antikoerper, Naalad antikoerper, putative N-acetylated-alpha-linked acidic dipeptidase antikoerper, folate hydrolase 1B antikoerper, folate hydrolase 1 antikoerper, folate hydrolase (prostate-specific membrane antigen) 1 antikoerper, LOC451185 antikoerper, FOLH1B antikoerper, FOLH1 antikoerper, Folh1 antikoerper

Hintergrund: Prostate specific antigen (PSA) is the most widely used marker of prostate cancer. Immunoassays for PSA using monoclonal (MAb) or polyclonal antibodies have clinical applications, such as monitoring and early detection of prostate cancer. However, PSA is not a perfect tumor marker because serum levels often are elevated in men with benign prostatic hyperplasia, prostatitis and other nonmalignant disorders and also because levels are not always elevated in individuals with early prostate cancer. 107-1A4 is a new prostate cancer-reactive MAb which can identify additional proteins that could be of value for the diagnosis and treatment of prostate cancer. It shows selective reactivity to prostate epithelial membrane antigens.