Kaninchen anti-Human IgD (Chain delta) Antikörper

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Antigen
Epitop
Chain delta
98
24
10
2
1
Reaktivität
Human
260
107
15
12
Wirt
Kaninchen
148
120
72
31
23
Klonalität
Polyklonal
Applikation
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Optionen
Hersteller
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Verwendungszweck This antibody is designed for the specific localization of human IgD using IHC techniques in formalin-fixed, paraffin- embedded tissue sections.
Immunogen IgD isolated from a pool of normal human sera.
Isotyp IgG
Produktmerkmale IgD makes up about 1 % of proteins in the plasma membranes of immature B-lymphocytes (coexpressed with IgM) and is also found in serum in very small amounts. It is monomeric and incorporates the alphaheavy chain in its structure. IgD's function is currently unknown, as mice lacking IgD seem to retain normal immune responses (implying redundancy if not lack of function), and IgD ceases to be expressed in activated B-lymphocytes. It may function as a regulatory antigen receptor. IgD antibody reacts with surface immunoglobulin IgD delta chains. This antibody is useful when identifying Leukemias, Plasmacytomas, and B-cell lineage derived from Lymphomas, specifically Marginal Zone Lymphoma. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Reinigung Concentrated
Antigen
Applikations-hinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Staining pattern: Cytoplasmic. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from tonsil.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for IgD.

Protokoll Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Testdurchführung

Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:25 - 1:100
Incubation:
3. min, RT
Control Tissue: Tonsil

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25 °C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C until the expiration date printed on product label.
Bilder des Herstellers
Immunohistochemistry (IHC) image for Rabbit anti-Human IgD (Chain delta) antibody (ABIN1027284) 10 × 20 × IHC of IgD (Polyclonal) on FFPE tonsil tissue. Citrate pH 6.5, DAB, Hematox...