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Kaninchen anti-Maus IgM (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
Produktnummer ABIN102684
  • Target Alle IgM Produkte
    IgM
    Bindungsspezifität
    • 141
    • 118
    • 87
    • 40
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 192
    • 133
    • 66
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    • 1
    Maus
    Wirt
    • 321
    • 187
    • 78
    • 19
    • 14
    • 5
    • 4
    • 1
    • 1
    Kaninchen
    Klonalität
    • 495
    • 87
    Polyklonal
    Konjugat
    • 120
    • 77
    • 76
    • 55
    • 36
    • 32
    • 24
    • 15
    • 12
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Applikation
    • 335
    • 249
    • 241
    • 209
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 47
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Spezifität
    IgM µ chain
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgM whole molecule

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product has been assayed against 1.0 μg of Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.5 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Aliquot to Avoid repeated freezing and thawing.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    IgM
    Abstract
    IgM Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: rabbit Anti-Mouse IgM (mu chain) Antibody Biotin Conjugated, rabbit Anti Mouse IgM mu chain Biotin Conjugated Antibody

    Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.

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