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Ziege anti-Maus IgM (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Produktnummer ABIN102675
  • Target Alle IgM Produkte
    IgM
    Bindungsspezifität
    • 141
    • 118
    • 87
    • 40
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 192
    • 133
    • 66
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    • 1
    Maus
    Wirt
    • 320
    • 188
    • 78
    • 19
    • 14
    • 5
    • 4
    • 1
    • 1
    Ziege
    Klonalität
    • 495
    • 87
    Polyklonal
    Konjugat
    • 120
    • 77
    • 76
    • 56
    • 35
    • 32
    • 24
    • 15
    • 12
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Alkaline Phosphatase (AP)
    Applikation
    • 335
    • 249
    • 241
    • 209
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 47
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Spezifität
    IgM µ chain
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    Preadsorption: Solid phase absorption
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Mouse IgM whole molecule

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Mouse IgM (mu chain) alkaline phosphatase conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

    ELISA Dilution: 1:4,500 - 1:8,000

    Western Blot Dilution: 1:500 - 1:2,500

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target
    IgM
    Abstract
    IgM Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: goat anti-Mouse IgM (Mu Chain) alkaline phosphatase Conjugated Antibody, goat anti-Mouse IgM Mu Chain Antibody alk phos Conjugation

    Background: Anti-Mouse IgM (mu chain) alkaline phosphatase conjugated antibody generated in goat detects specifically mouse IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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