Ziege anti-Affe IgM (Chain mu) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN102652

Produktdetails

Target: IgM

Bindungsspezifität: Chain mu

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgM µ chain

Kreuzreaktivität: Affe

Produktmerkmale: Anti-Monkey IgM (mu chain) biotin conjugated antibody generated in goat detects specifically monkey IgM (mu chain). This secondary biotin conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Monkey IgM whole molecule

Isotyp: IgM

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Monkey IgG (mu chain) Biotin conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,000 - 1:300,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Referenzen

Miller-Novak, Das, Musich, Demberg, Weiner, Venzon, Mohanram, Vargas-Inchaustegui, Tuero, Ackerman, Alter, Robert-Guroff: "Analysis of Complement-Mediated Lysis of Simian Immunodeficiency Virus (SIV) and SIV-Infected Cells Reveals Sex Differences in Vaccine-Induced Immune Responses in Rhesus Macaques." in: Journal of virology, Vol. 92, Issue 19, (2018) (PubMed).

Antigendetails

Target: IgM

Abstract: IgM Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat Anti-Monkey IgM Antibody biotin Conjugation, Goat Anti-Monkey IgM biotin Conjugated Antibody

Background: Anti-Monkey IgM (mu chain) biotin conjugated antibody generated in goat detects specifically monkey IgM (mu chain). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.