Kaninchen anti-Schaf IgG (Fc Region) Antikörper - Preadsorbed

Produktnummer ABIN102286

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG F(c)

Kreuzreaktivität: Schaf

Produktmerkmale: Anti-Sheep IgG F(c) antibody generated in rabbit detects specifically sheep IgG F(c). Anti-Sheep IgG F(c) secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Sheep IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: immunoaffinity chromatography using Sheep IgG coupled to agarose beads

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Sheep IgG F(c) antibody has been assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:10,000 - 1:200,000

Western Blot Dilution: 1:1,000 - 1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody

Background: Anti-Sheep IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of sheep IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Sheep IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.