Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Kaninchen
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Alkaline Phosphatase (AP)
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- IgG (H&L)
- Produktmerkmale
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Anti Rabbit Conjugated Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.
Concentration Definition: by UV absorbance at 280 nm - Aufreinigung
- Preadsorption: Solid phase absorption
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: Rabbit IgG whole molecule
- Isotyp
- IgG
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:500 - 1:2,500
- Kommentare
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Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Lagerung
- 4 °C
- Haltbarkeit
- 12 months
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Resveratrol attenuates denervation-induced muscle atrophy due to the blockade of atrogin-1 and p62 accumulation." in: International journal of medical sciences, Vol. 15, Issue 6, pp. 628-637, (2018) (PubMed).
: "
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Resveratrol attenuates denervation-induced muscle atrophy due to the blockade of atrogin-1 and p62 accumulation." in: International journal of medical sciences, Vol. 15, Issue 6, pp. 628-637, (2018) (PubMed).
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation, goat anti-Rabbit IgG Alk Phos Conjugated Antibody
Background: Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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