Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper

Details zu Produkt Nr. ABIN102001, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6012
1904
1869
1159
274
267
266
124
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reaktivität
Kaninchen
2740
2674
2191
1495
1370
700
664
447
434
362
359
354
275
258
257
147
115
84
43
26
24
21
12
12
8
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
Wirt
Ziege
6468
4816
1755
887
458
274
87
54
43
35
22
8
6
5
4
1
1
1
Klonalität
Polyklonal
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen

Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.

Immunogen Type: Native Protein

Isotyp IgG
Spezifität IgG (H&L)
Kreuzreaktivität Kaninchen
Produktmerkmale Anti-Rabbit IgG antibody generated in goat detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Reinigung This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.  Specificity was confirmed using ELISA against at less than 1% of target signal.
Sterilität Sterile filtered
Antigen
Hintergrund

Synonyms: goat anti-Rabbit IgG Antibody, goat anti-Rabbit IgG Antibody pre-adsorbed, Rabbit Secondary Antibody

Background: Anti-Rabbit IgG (H&L) Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Applikations-hinweise

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-Rabbit IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:15,000 - 1:30,000

Western Blot Dilution: 1:500 - 1:2,000

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (ABIN102001) Western Blot of Goat anti-RABBIT IgG (H&L) Antibody Pre-adsorbed. Lane 1: Rabbit IgG....
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (ABIN102001) Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs,...
SDS-PAGE (SDS) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (ABIN102001) SDS-Page of Goat anti-Rabbit IgG (H&L) Pre-adsorbed Secondary Antibody. Lane 1: Goat ...
Allgemeine Veröffentlichungen Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).