Ziege anti-Schwein IgG (Heavy & Light Chain) Antikörper (FITC)

Details zu Produkt Nr. ABIN101878, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6022
1904
1862
1159
269
266
261
124
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reaktivität
Schwein
2738
2669
2179
1495
1361
698
661
445
429
362
355
350
275
261
257
149
115
84
43
26
24
21
12
12
8
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
Wirt
Ziege
6485
4765
1756
888
459
274
81
54
43
35
22
8
5
4
1
1
1
Klonalität
Polyklonal
Konjugat
FITC
2050
1734
1623
1155
678
643
422
316
171
122
102
102
94
89
88
88
86
84
79
78
74
73
68
68
68
67
65
65
61
55
54
54
54
54
54
53
53
53
53
53
53
48
47
47
41
40
32
31
29
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
10
10
10
10
10
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen

Immunogen: Swine IgG whole molecule

Isotyp IgG
Spezifität IgG (H&L)
Produktmerkmale Concentration Definition: by UV absorbance at 280 nm
Reinigung This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Swine IgG and Swine Serum.
Labeling Ratio 3.64
Antigen
Hintergrund

Synonyms: goat Anti-Swine IgG fluorescein Conjugated Antibody, goat Anti-Swine IgG Antibody FITC Conjugation

Background: Anti-Swine IgG (H&L) Fluorescein Antibody generated in goat detects reactivity to swine IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG Antibody is conjugated to Fluorescein.

Applikations-hinweise

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Kommentare

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Product is photosensitive and should be protected from light.
Lagerung RT,4 °C,-20 °C
Haltbarkeit 12 months
Bilder des Herstellers
 image for Goat anti-Pig (Porcine) IgG (Heavy & Light Chain) antibody (FITC) (ABIN101878) Goat anti-Pig (Porcine) IgG (Heavy & Light Chain) antibody (FITC)
 image for Goat anti-Pig (Porcine) IgG (Heavy & Light Chain) antibody (FITC) (ABIN101878) Goat anti-Pig (Porcine) IgG (Heavy & Light Chain) antibody (FITC) (Image 2)
Allgemeine Veröffentlichungen The, Feltkamp: "Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation." in: Immunology, Vol. 18, Issue 6, pp. 865-73, 1970 (PubMed).