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Kaninchen anti-Maus IgG (Heavy & Light Chain) Antikörper - Preadsorbed

ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Produktnummer ABIN101785
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 694
    • 672
    • 561
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 1730
    • 1473
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Kaninchen
    Klonalität
    • 3360
    • 167
    • 1
    Polyklonal
    Applikation
    • 1839
    • 1800
    • 1791
    • 1500
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag)
    Spezifität
    IgG (H&L)
    Kreuzreaktivität
    Maus
    Produktmerkmale
    Anti-Mouse antibody generated in rabbit detects specifically mouse IgG (H&L). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    Preadsorption: Solid phase absorption
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Anti-Mouse IgG was produced by repeated immunization with mouse IgG whole molecule in rabbit

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise
    The rabbit anti-mouse IgG antibody ABIN101785 is suitable for use in ELISA, immunohistochemistry, and Western Blot, CUT&RUN and CUT&Tag. Specific conditions for each assay should be optimized by the end user. General ABIN101785 dilution recommendations for different applications are as follows:
    • ELISA: 1:20,000 - 1:40,000
    • WB: 1:2,000 - 1:10,000
    • IHC: 1:1,000 - 1:5,000
    • CUT&RUN: 1:100
    • CUT&Tag: 1:100
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Haltbarkeit
    12 months
  • Fujiwara, Tanno, Sugishita, Kishi, Makino, Okada: "Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag." in: PLoS ONE, Vol. 16, Issue 11, pp. e0259846, (2021) (PubMed).

    Willcockson, Healton, Weiss, Bartholdy, Botbol, Mishra, Sidhwani, Wilson, Pinto, Maron, Skalina, Toro, Zhao, Lee, Hou, Yusufova, Meydan, Osunsade, David, Cesarman, Melnick, Sidoli, Garcia, Edelmann et al.: "H1 histones control the epigenetic landscape by local chromatin compaction. ..." in: Nature, (2020) (PubMed).

    Vonk, van Dooremalen, Liv, Klumperman, Coffer, Saris, Lorenowicz: "Mesenchymal Stromal/stem Cell-derived Extracellular Vesicles Promote Human Cartilage Regeneration In Vitro." in: Theranostics, Vol. 8, Issue 4, pp. 906-920, (2019) (PubMed).

    Kooijmans, Gitz-Francois, Schiffelers, Vader: "Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach." in: Nanoscale, Vol. 10, Issue 5, pp. 2413-2426, (2019) (PubMed).

  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: rabbit anti-Mouse IgG Antibody, Rabbit-a-Mouse IgG (H&L), Mouse IgG Antibody in rabbit

    Background: Anti-Mouse IgG antibody generated in rabbit detects specifically mouse IgG (H&L). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This anti-Mouse secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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