Ziege anti-Esel IgG (Fc Region) Antikörper (FITC)
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Fc Region
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Reaktivität
- Esel
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- FITC
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Applikation
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Spezifität
- IgG F(c)
- Kreuzreaktivität
- Esel
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Donkey IgG, Donkey IgG F(c) and Donkey Serum. No reaction occurred against Donkey IgG F(ab).
- Immunogen
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Immunogen: Donkey IgG F(c) fragment
- Isotyp
- IgG
- Labeling Ratio
- 3.4
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Donkey anti-Donkey IgG (Heavy & Light Chain) antibody (PE) - Preadsorbed
FACS, FM Polyclonal IgG PE
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- Applikationshinweise
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Kommentare
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Excitation/Emission wavelength: 494 nm/514 nm
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Product is photosensitive and should be protected from light.
- Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Goat anti-Donkey IgG F(c) fragment FITC conjugated Antibody, Goat anti-Donkey IgG Fc Fluorescein Conjugated antibody
Background: Anti-Donkey IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of donkey IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
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