Kaninchen anti-Esel IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Esel
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Alkaline Phosphatase (AP)
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- IgG (H&L)
- Kreuzreaktivität
- Esel
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- Preadsorption: Solid phase absorption
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: Donkey IgG whole molecule
- Isotyp
- IgG
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: This product has been assayed against 1.0 μg of Donkey IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate code # NPP-10) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:15,000
Western Blot Dilution: 1:500 - 1:2,500
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Lagerung
- 4 °C
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: rabbit anti-Donkey IgG Antibody alkaline phosphatase Conjugation, rabbit anti-Donkey IgG alk phos Conjugated Antibody
Background: Anti-Donkey IgG Alkaline Phosphatase Antibody generated in rabbit detects donkey IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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