Kaninchen anti-Huhn IgG (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN101035

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG (H&L)

Kreuzreaktivität: Huhn

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Chicken IgY / IgG whole molecule was produced by repeated immunization with Chicken IgY / IgG whole molecule in rabbit.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Secondary antibodies are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods

ELISA Dilution: 1:500,000

Western Blot Dilution: 1:2,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Rabbit anti-Chicken IgG Biotin Conjugated Antibody, Rabbit anti-Chicken IgG Antibody Biotin Conjugation, Chicken Secondary Antibody

Background: Anti-Chicken IgG Biotin Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.