Ziege anti-Huhn IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Huhn
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Alkaline Phosphatase (AP)
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- IgG (H&L)
- Kreuzreaktivität
- Huhn
- Produktmerkmale
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Anti-Chicken IgG whole molecule antibody generated in goat detects specifically Chicken IgG whole molecule. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - Aufreinigung
- Preadsorption: immunoaffinity chromatography using Chicken IgG coupled to agarose beads
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: Anti-Chicken IgG whole molecule was produced by repeated immunization with Chicken IgG whole molecule in goat.
Immunogen Type: Native Protein
- Isotyp
- IgG
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Chicken IgG whole molecule is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,500
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Lagerung
- 4 °C
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Chicken Antibody, Gt-a-Chicken IgG, Chicken Antibody in Goat, Chicken Secondary Antibody
Background: Anti-Chicken IgG Alkaline Phosphatase Antibody generated in goat detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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