Kaninchen anti-Rind (Kuh) IgG (Fc Region) Antikörper (Biotin) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Fc Region
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Reaktivität
- Rind (Kuh)
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Biotin
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- IgG F(c)
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- Preadsorption: immunoaffinity chromatography using Bovine IgG coupled to agarose beads
- Immunogen
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Immunogen: Bovine IgG F(c) fragment
- Isotyp
- IgG
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Rabbit anti-Cow IgG (Fc Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:15,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.5 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Aliquot to Avoid repeated freezing and thawing.
- Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: rabbit anti Bovine IgG F(c) Antibody Biotin Conjugated, rabbit anti Bovine IgG Fc Antibody Biotin Conjugated, rabbit anti Bovine IgG Fc fragment biotin conjugate
Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
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