Ziege anti-Human IgA (Heavy Chain) Antikörper (Biotin)

Details zu Produkt Nr. ABIN100717
Antigen
Epitop
Heavy Chain
48
7
Reaktivität
Human
28
12
7
7
7
1
1
Wirt
Ziege
44
14
3
1
Klonalität
Polyklonal
Konjugat
Biotin
9
7
7
7
6
6
6
2
2
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Optionen
Immunogen

Immunogen: Anti-Human IgA alpha heavy chain antibody was produced by repeated immunization with Human IgA alpha heavy chain in goat.

Immunogen Type: Native Protein

Isotyp IgG
Kreuzreaktivität Human
Produktmerkmale Anti-Human IgA alpha heavy chain antibody generated in goat detects specifically Human IgA alpha heavy chain.
Concentration Definition: by UV absorbance at 280 nm
Reinigung Anti-HUMAN IgA (alpha chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Human IgA and Human Serum.  No reaction was observed against other Human heavy or light chain proteins.
Labeling Ratio 10-20
Antigen
Hintergrund

Synonyms: goat anti-Human IgA (alpha chain) biotin conjugated Antibody, goat anti-Human IgA alpha Antibody biotin conjugation

Background: Anti-Human IgA Biotin Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Applikations-hinweise

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-HUMAN IgA (alpha chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:60,000 - 1:600,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Aliquot to Avoid repeated freezing and thawing.
Lagerung RT,4 °C,-20 °C
Haltbarkeit 12 months
Produkt verwendet in: Yu, Pollock, Duval, Lewis, Joseph, Meade, Cavacini: "Neutralization of HIV by milk expressed antibody." in: Journal of acquired immune deficiency syndromes (1999), Vol. 62, Issue 1, pp. 10-6, 2013 (PubMed).

Allgemeine Veröffentlichungen Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).