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Ziege anti-Human IgA (Heavy Chain) Antikörper (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
Produktnummer ABIN100717
  • Target Alle IgA Produkte
    IgA
    Bindungsspezifität
    • 104
    • 60
    • 38
    • 15
    • 15
    • 13
    • 8
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain
    Reaktivität
    • 159
    • 58
    • 44
    • 16
    • 16
    • 14
    • 14
    • 13
    • 13
    • 12
    • 10
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Human
    Wirt
    • 200
    • 98
    • 49
    • 17
    • 12
    • 6
    • 2
    Ziege
    Klonalität
    • 307
    • 62
    Polyklonal
    Konjugat
    • 87
    • 52
    • 49
    • 37
    • 28
    • 26
    • 8
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Applikation
    • 180
    • 135
    • 129
    • 111
    • 100
    • 96
    • 56
    • 40
    • 39
    • 29
    • 28
    • 24
    • 22
    • 18
    • 17
    • 17
    • 17
    • 7
    • 7
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Kreuzreaktivität
    Human
    Produktmerkmale
    Anti-Human IgA alpha heavy chain antibody generated in goat detects specifically Human IgA alpha heavy chain.
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Anti-Human IgA alpha heavy chain antibody was produced by repeated immunization with Human IgA alpha heavy chain in goat.

    Immunogen Type: Native Protein

    Isotyp
    IgG
    Labeling Ratio
    10-20
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-HUMAN IgA (alpha chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:60,000 - 1:600,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Aliquot to Avoid repeated freezing and thawing.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Yu, Pollock, Duval, Lewis, Joseph, Meade, Cavacini: "Neutralization of HIV by milk expressed antibody." in: Journal of acquired immune deficiency syndromes (1999), Vol. 62, Issue 1, pp. 10-6, (2013) (PubMed).

  • Target
    IgA
    Abstract
    IgA Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: goat anti-Human IgA (alpha chain) biotin conjugated Antibody, goat anti-Human IgA alpha Antibody biotin conjugation

    Background: Anti-Human IgA Biotin Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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