TRIM5 Antikörper (Tripartite Motif Containing 5)

Details for Product anti-TRIM5 Antibody No. ABIN1003379, Anbieter: Anmelden zum Anzeigen
Antigen
  • TRIM5
  • RNF88
  • TRIM5alpha
  • EG667823
  • Gm8833
  • RGD1304579
  • tripartite motif containing 5
  • tripartite motif-containing 5
  • TRIM5
  • Trim5
Reaktivität
Human
140
7
6
1
1
1
Wirt
Kaninchen
91
38
13
Klonalität
Polyklonal
Konjugat
Dieser TRIM5 Antikörper ist unkonjugiert
5
5
5
4
4
4
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
118
98
63
38
11
7
6
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Rabbit polyclol TRIM5delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5( (Genbank accession No. NP_149084).
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN1004486.
Isotyp IgG
Reinigung Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmide, Primers & weitere TRIM5 products on genomics-online (e.g. as negative or positive controls)
Antigen Tripartite Motif Containing 5 (TRIM5)
Andere Bezeichnung TRIM5delta (Retroviral Restriction Factor) (TRIM5 Antibody Abstract)
Hintergrund TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha-variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. The TRIM5delta isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5delta also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.
Pathways Activation of Innate immune Response
Applikations-hinweise TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 1 to 2 µg/ml. (Optimal dilution should be determined by user.) A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Lagerung 4 °C
Haltbarkeit 12 months
Produkt verwendet in: Hatziioannou, Perez-Caballero, Yang, Cowan, Bieniasz: "Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 29, pp. 10774-9, 2004 (PubMed).

Stremlau, Owens, Perron, Kiessling, Autissier, Sodroski: "The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys." in: Nature, Vol. 427, Issue 6977, pp. 848-53, 2004 (PubMed).

Xu, Yang, Moitra, Hashimoto, Rallabhandi, Kaul, Meroni, Jensen, Weissman, DArpa: "BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta." in: Experimental cell research, Vol. 288, Issue 1, pp. 84-93, 2003 (PubMed).

Allgemeine Veröffentlichungen Reymond, Meroni, Fantozzi, Merla, Cairo, Luzi, Riganelli, Zanaria, Messali, Cainarca, Guffanti, Minucci, Pelicci, Ballabio: "The tripartite motif family identifies cell compartments." in: The EMBO journal, Vol. 20, Issue 9, pp. 2140-51, 2001 (PubMed).

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