TRIM5 Antikörper (Tripartite Motif Containing 5) (C-Term)

Details for Product anti-TRIM5 Antibody No. ABIN1003377, Anbieter: Anmelden zum Anzeigen
Antigen
  • TRIM5
  • RNF88
  • TRIM5alpha
  • EG667823
  • Gm8833
  • RGD1304579
  • tripartite motif containing 5
  • tripartite motif-containing 5
  • TRIM5
  • Trim5
Epitop
C-Term
29
20
11
9
7
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human
140
7
6
1
1
1
Wirt
Kaninchen
91
38
13
Klonalität
Polyklonal
Konjugat
Dieser TRIM5 Antikörper ist unkonjugiert
5
5
5
4
4
4
Applikation
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
117
98
62
38
11
7
6
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Rabbit polyclol TRIM5alpha antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5( (Genbank accession No. AAS48505).
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN1003907.
Isotyp IgG
Reinigung Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmide, Primers & weitere TRIM5 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung TRIM5alpha (Retroviral Restriction Factor) (TRIM5 Antibody Abstract)
Hintergrund TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha-variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5alpha variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5alpha acts as a natural barrier to cross-species retrovirus transmission.
Pathways Activation of Innate immune Response
Applikations-hinweise TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 1 to 2 µg/ml. (Optimal dilution should be determined by user.) A band at approximately 55 kDa can be detected. Antibody can also be used for immunofluorescence microscopy and ELISA and might be suited for other applications not tested so far. Anti-TRIM5 alpha is human specific but should presumably also react with rhesus protein.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Lagerung 4 °C
Haltbarkeit 12 months
Produkt verwendet in: Hatziioannou, Perez-Caballero, Yang, Cowan, Bieniasz: "Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 29, pp. 10774-9, 2004 (PubMed).

Stremlau, Owens, Perron, Kiessling, Autissier, Sodroski: "The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys." in: Nature, Vol. 427, Issue 6977, pp. 848-53, 2004 (PubMed).

Allgemeine Veröffentlichungen Reymond, Meroni, Fantozzi, Merla, Cairo, Luzi, Riganelli, Zanaria, Messali, Cainarca, Guffanti, Minucci, Pelicci, Ballabio: "The tripartite motif family identifies cell compartments." in: The EMBO journal, Vol. 20, Issue 9, pp. 2140-51, 2001 (PubMed).

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