GFP Antikörper (AA 246) (Texas Red (TR))

Produktnummer ABIN100090

Produktdetails anti-GFP Antikörper (Texas Red (TR))

Target: GFP (Green Fluorescent Protein (GFP))

Bindungsspezifität: AA 246

Reaktivität: Aequorea victoria

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit Texas Red (TR)

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-GFP Antikörper (Texas Red (TR)) (ABIN100090)

Gupta, Verfaillie, Chmielewski, Kren, Eidman, Connaire, Heremans, Lund, Blackstad, Jiang, Luttun, Rosenberg: "Isolation and characterization of kidney-derived stem cells." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 11, pp. 3028-40, (2007) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP (GFP Produkte)

Synonyme: green fluorescent protein antikoerper, gfp antikoerper

Substanzklasse: Viral Protein

Hintergrund: GFP Texas Red™ Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.