anti-Human Heat Shock 60kDa Protein 1 (Chaperonin) Antikörper für Western Blotting

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
  • CPN60
  • GROEL
  • HLD4
  • HSP-60
  • HSP60
  • HSP65
  • HuCHA60
  • SPG13
  • chaperonin
  • cpn60
  • groel
  • hld4
  • hsp60
  • hsp65
  • spg13
  • 60kDa
  • Hsp60
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • d-hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • Hspd1-30p
  • heat shock protein family D (Hsp60) member 1
  • heat shock protein family D (Hsp60) member 1 S homeolog
  • 60 kDa heat shock protein, mitochondrial
  • heat shock protein 1 (chaperonin)
  • Heat shock protein 60A
  • heat shock 60 protein 1
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • thermosome subunit
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein family D member 1
  • HSPD1
  • hspd1.S
  • hspd1
  • LOC100414401
  • Hspd1
  • Hsp60A
  • HSP60
  • groEL
  • MMP_RS07785
  • groEl
  • LOC100136430
Reaktivität
Bakterien, Huhn, Rind (Kuh), Hund, Fisch, Meerschweinchen, Hamster, Human, Insekten, Affe, Maus, Schwein, Pflanzen, Kaninchen, Ratte (Rattus), Trichinella spiralis, Yeast (Saccharomyces cerevisiae)
826
563
548
387
342
325
317
303
278
269
192
180
132
119
115
104
77
58
39
38
37
36
35
35
22
21
20
19
18
18
18
17
17
6
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Maus
562
337
10
Klonalität
Monoklonal
Konjugat
Unkonjugiert
35
28
27
27
23
22
17
12
12
12
12
12
12
12
12
12
12
11
11
11
11
11
10
10
9
9
9
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
3
Applikation
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
762
434
407
371
318
270
232
184
85
63
42
41
8
7
6
6
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN361786
$ 198.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.168048 ABIN3044334 ICC IHC (p) WB Rabbit IgG AA 503-516, C-Term Anmelden zum Anzeigen Polyclonal 4
3.168048 ABIN3043854 ICC IHC (p) WB Rabbit IgG AA 260-496 Anmelden zum Anzeigen Polyclonal 4
3.168048 ABIN2486159 IP IHC ELISA WB FITC Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486148 IP IHC ELISA WB Atto 390 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486151 IP IHC ELISA WB Atto 594 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486150 IP IHC ELISA WB Atto 565 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486153 IP IHC ELISA WB Atto 655 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486160 IP IHC ELISA WB HRP Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486158 IP IHC ELISA WB Biotin Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486161 IP IHC ELISA WB PE-Atto 594 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486163 IP IHC ELISA WB PE Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486162 IP IHC ELISA WB PerCP Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486149 IP IHC ELISA WB Atto 488 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486154 IP IHC ELISA WB Atto 680 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486152 IP IHC ELISA WB Atto 633 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486156 IP IHC ELISA WB Alkaline Phosphatase (AP) Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486164 IP IHC ELISA WB Streptavidin Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486155 IP IHC ELISA WB Atto 700 Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2486157 IP IHC ELISA WB APC Rabbit Anmelden zum Anzeigen Polyclonal 12
3.168048 ABIN2788677 WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
Reaktivität Bakterien, Huhn, Rind (Kuh), Hund, Fisch, Meerschweinchen, Hamster, Human, Insekten, Affe, Maus, Schwein, Pflanzen, Kaninchen, Ratte (Rattus), Trichinella spiralis, Yeast (Saccharomyces cerevisiae)
(826), (563), (548), (387), (342), (325), (317), (303), (278), (269), (192), (180), (132), (119), (115), (104), (77), (58), (39), (38), (37), (36), (35), (35), (22), (21), (20), (19), (18), (18), (18), (17), (17), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Wirt Maus
(562), (337), (10)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Unkonjugiert
(35), (28), (27), (27), (23), (22), (17), (12), (12), (12), (12), (12), (12), (12), (12), (12), (12), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3)
Applikation Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(762), (434), (407), (371), (318), (270), (232), (184), (85), (63), (42), (41), (8), (7), (6), (6), (2), (2), (1), (1)
Pubmed 16 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Spezifität Detects ~60 kDa.
Reinigung Protein G Purified
Immunogen Recombinant human HSP60
Klon LK2
Isotyp IgG1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung HSP60 (HSPD1 Antibody Abstract)
Hintergrund In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
Gen-ID 3329
NCBI Accession NP_002147
UniProt P10809
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikations-hinweise
  • WB (1:4000)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Kommentare

0.25 μg/ml of SMC-111 was sufficient for detection of HSP60 in 10 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.1 mM PMSF
Lagerung -20 °C

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Teigen, Orczewska, McLaughlin, OBrien: "Cold acclimation increases levels of some heat shock protein and sirtuin isoforms in threespine stickleback." in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol. 188, pp. 139-47, 2015

Bayot, Gareil, Rogowska-Wrzesinska, Roepstorff, Friguet, Bulteau: "Identification of novel oxidized protein substrates and physiological partners of the mitochondrial ATP-dependent Lon-like protease Pim1." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11445-57, 2010

Allgemeine Veröffentlichungen

Verda, Kim, Ikehara, Statkute, Bronesky, Petrenko, Oyama, He, Link, Vahanian, Burt: "Hematopoietic mixed chimerism derived from allogeneic embryonic stem cells prevents autoimmune diabetes mellitus in NOD mice." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 2, pp. 381-6, 2008

Lai, Zhuang, Zhang: "[Stability of implants placed in different bone types]" in: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology, Vol. 42, Issue 5, pp. 292-3, 2007

Lai, Liu, Ting, Yang, Huang, Wallace, Kaiser, Wang: "Regulation of IGF-I receptor signaling in diabetic cardiac muscle: dysregulation of cytosolic and mitochondria HSP60." in: American journal of physiology. Endocrinology and metabolism, Vol. 292, Issue 1, pp. E292-7, 2007

Deocaris, Kaul, Wadhwa: "On the brotherhood of the mitochondrial chaperones mortalin and heat shock protein 60." in: Cell stress & chaperones, Vol. 11, Issue 2, pp. 116-28, 2006

Gupta, Knowlton: "HSP60, Bax, apoptosis and the heart." in: Journal of cellular and molecular medicine, Vol. 9, Issue 1, pp. 51-8, 2005

Bason, Corrocher, Lunardi, Puccetti, Olivieri, Girelli, Navone, Beri, Millo, Margonato, Martinelli, Puccetti: "Interaction of antibodies against cytomegalovirus with heat-shock protein 60 in pathogenesis of atherosclerosis." in: Lancet (London, England), Vol. 362, Issue 9400, pp. 1971-7, 2003

Hartl, Hayer-Hartl: "Molecular chaperones in the cytosol: from nascent chain to folded protein." in: Science (New York, N.Y.), Vol. 295, Issue 5561, pp. 1852-8, 2002

Itoh, Komatsuda, Ohtani, Wakui, Imai, Sawada, Otaka, Ogura, Suzuki, Hamada: "Mammalian HSP60 is quickly sorted into the mitochondria under conditions of dehydration." in: European journal of biochemistry / FEBS, Vol. 269, Issue 23, pp. 5931-8, 2002

LaVerda, Kalayoglu, Byrne: "Chlamydial heat shock proteins and disease pathology: new paradigms for old problems?" in: Infectious diseases in obstetrics and gynecology, Vol. 7, Issue 1-2, pp. 64-71, 1999

Bukau, Horwich: "The Hsp70 and Hsp60 chaperone machines." in: Cell, Vol. 92, Issue 3, pp. 351-66, 1998

Neuer, Lam, Tiller, Kiesel, Witkin: "Humoral immune response to membrane components of Chlamydia trachomatis and expression of human 60 kDa heat shock protein in follicular fluid of in-vitro fertilization patients." in: Human reproduction (Oxford, England), Vol. 12, Issue 5, pp. 925-9, 1997

Hartl: "Molecular chaperones in cellular protein folding." in: Nature, Vol. 381, Issue 6583, pp. 571-9, 1996

Gao, Brosnan, Raine: "Experimental autoimmune encephalomyelitis. Qualitative and semiquantitative differences in heat shock protein 60 expression in the central nervous system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3548-56, 1995

Jindal, Dudani, Singh, Harley, Gupta: "Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen." in: Molecular and cellular biology, Vol. 9, Issue 5, pp. 2279-83, 1989

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
 image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN361786) Hsp60 (LK 2), Listeria infected mice spleens 6 days after infection with L monocytoge...
Western Blotting (WB) image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN361786) Hsp60 WB 1 in 1000 HS Hela 10ug.