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General |
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Antigen | Angiotensin II Receptor, Type 2 (AGTR2) Antikörper |
Epitop | Internal Region Alternativen |
Reaktivität | Fledermaus, Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus), Schaf Alternativen |
Wirt | Kaninchen Alternativen |
Klonalität | |
Konjugat | Dieser Angiotensin II Type 2 Receptor Antikörper ist unkonjugiert Alternativen |
Applikation |
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternativen
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Hersteller | Anmelden zum Anzeigen |
Produktdetails anti-Angiotensin II Type 2 Receptor AntikörperTarget Details Angiotensin II Type 2 Receptor Anwendungsinformationen Handhabung Bilder |
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Marke | IHC-plus™ |
Spezifität | Human AGTR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR65 (63 % ), NPFFR2 (44 %). |
Homologie | Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%). |
Reinigung | Immunoaffinity purified |
Immunogen |
Synthetic 16 amino acid peptide from internal region of human AGTR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%). Type of Immunogen: Synthetic peptide |
Plasmids, Primers & others | |
Target Details Angiotensin II Type 2 ReceptorProduktdetails anti-Angiotensin II Type 2 Receptor Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | AGTR2 / AT2 Receptor (AGTR2 Antibody Abstract) |
Hintergrund |
Name/Gene ID: AGTR2 Subfamily: Angiotensin Family: GPCR Synonyms: AGTR2, Angiotensin ii receptor type 2, Angiotensin II type-2 receptor, AT2 receptor, AT2, At2r, MRX88, Angiotensin ii at2, Angiotensin receptor 2, Type-2 angiotensin II receptor, ATGR2 |
Gen-ID | 186 |
Pathways | ACE Inhibitor Pathway, Hormone Transport, Regulation of Systemic Arterial Blood Pressure by Hormones |
AnwendungsinformationenProduktdetails anti-Angiotensin II Type 2 Receptor Antikörper Target Details Angiotensin II Type 2 Receptor Handhabung Bilder zurück nach oben |
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Applikations-hinweise |
Approved: IHC, IHC-P (10 μg/mL) Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. |
Kommentare |
Target Species of Antibody: Human |
Testdurchführung |
The IHC-pro Immunohistochemistry Protocol Tissue Preparation Formalin fixation and embedding in paraffin wax Tissue Sectioning Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C Deparaffinization Wash slides in 3 changes of xylene – 5 minutes each at room temperature. Rehydration Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature. Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature. Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature. Rinse slides in gentle running distilled water – 5 minutes at room temperature. Antigen retrieval Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature. Immunostaining Do not allow tissues to dry at any time during the staining procedure. Apply a universal protein block – 20 minutes at room temperature. Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature. Rinse slides 1X TBST – 1 minute at room temperature. Apply alkaline phosphatase streptavidin – 30 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature. Wash slides in distilled water – 1 minute at room temperature. Dehydrate This method should only be used if the chromogen substrate is alcohol insoluble. Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature. Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of xylene – 1 minute each at room temperature. Apply coverslip |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-Angiotensin II Type 2 Receptor Antikörper Target Details Angiotensin II Type 2 Receptor Anwendungsinformationen Bilder zurück nach oben |
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Format | Liquid |
Konzentration | Lot specific |
Buffer | PBS, less than 0.1 % sodium azide. |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Lagerung | 4 °C,-20 °C |
Informationen zur Lagerung | Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles. |
Haltbarkeit | 12 months |
BilderProduktdetails anti-Angiotensin II Type 2 Receptor Antikörper Target Details Angiotensin II Type 2 Receptor Anwendungsinformationen Handhabung zurück nach oben |
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Bilder des Herstellers |