anti-Affe Angiotensin II Type 2 Receptor Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended Angiotensin II Type 2 Receptor Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Angiotensin II Receptor, Type 2 (AGTR2) Antikörper
  • AT2
  • ATGR2
  • MRX88
  • AI316812
  • AW107640
  • AT2-R
  • AT2R
  • AT2R
  • at2
  • AGTR2
  • angiotensin II receptor type 2
  • angiotensin II receptor, type 2
  • AGTR2
  • Agtr2
Epitop
Internal Region
20
10
6
4
3
1
Reaktivität
Fledermaus, Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus), Schaf
72
30
29
2
2
2
2
1
1
Wirt
Kaninchen
49
21
2
Klonalität
Polyklonal
Konjugat
Dieser Angiotensin II Type 2 Receptor Antikörper ist unkonjugiert
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
39
32
22
21
20
15
14
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1048323
$ 484.00
Zzgl. Versandkosten $45.00

General

Antigen Angiotensin II Receptor, Type 2 (AGTR2) Antikörper
Epitop Internal Region
(20), (10), (6), (4), (3), (1)
Reaktivität Fledermaus, Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus), Schaf
(72), (30), (29), (2), (2), (2), (2), (1), (1)
Wirt Kaninchen
(49), (21), (2)
Klonalität
Polyklonal
Konjugat Dieser Angiotensin II Type 2 Receptor Antikörper ist unkonjugiert
(3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(39), (32), (22), (21), (20), (15), (14), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Angiotensin II Type 2 Receptor Antikörper

Target Details Angiotensin II Type 2 Receptor Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human AGTR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR65 (63 % ), NPFFR2 (44 %).
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human AGTR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%).

Type of Immunogen: Synthetic peptide

Target Details Angiotensin II Type 2 Receptor

Produktdetails anti-Angiotensin II Type 2 Receptor Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung AGTR2 / AT2 Receptor (AGTR2 Antibody Abstract)
Hintergrund Name/Gene ID: AGTR2
Subfamily: Angiotensin
Family: GPCR

Synonyms: AGTR2, Angiotensin ii receptor type 2, Angiotensin II type-2 receptor, AT2 receptor, AT2, At2r, MRX88, Angiotensin ii at2, Angiotensin receptor 2, Type-2 angiotensin II receptor, ATGR2
Gen-ID 186
Pathways ACE Inhibitor Pathway, Hormone Transport, Regulation of Systemic Arterial Blood Pressure by Hormones

Anwendungsinformationen

Produktdetails anti-Angiotensin II Type 2 Receptor Antikörper Target Details Angiotensin II Type 2 Receptor Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
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Handhabung

Produktdetails anti-Angiotensin II Type 2 Receptor Antikörper Target Details Angiotensin II Type 2 Receptor Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-Angiotensin II Type 2 Receptor Antikörper Target Details Angiotensin II Type 2 Receptor Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Angiotensin II Receptor, Type 2 (AGTR2) (Internal Region) antibody (ABIN1048323) Anti-AGTR2 antibody ABIN1048323 IHC staining of human adrenal. Immunohistochemistry ...
Immunohistochemistry (IHC) image for anti-Angiotensin II Receptor, Type 2 (AGTR2) (Internal Region) antibody (ABIN1048323) Anti-AGTR2 antibody ABIN1048323 IHC staining of human uterus. Immunohistochemistry o...