Cell Culture Supplements

Cell cultures are one of the major techniques in life sciences frequently used in research, development and production. Cells, tissues or organs are harvested in order to proliferate in an artificial environment. Cell cultures supplements are required for optimal cell expansion. Scientists have to mimic the in vivo conditions like controlled temperature or a substrate for cell attachment. Especially difficult to cultivate cells need appropriate growth medium and incubator that maintains correct pH and osmolality for the in vitro cultivation.

Human Platelet Lysate: A new Era of Cell Culture Supplements

antibodies-online offers Human Platelet Lysates cell culture supplement that is a first-rate alternative to fetal bovine serum (FBS) in human cell culture medium. In contrast to FBS, Human blood offers abundant growth factors. hPL enables higher proliferation rates in various cell lines, cutting the population doubling time in half(Fig.1).1

Note: Revolutionize your matrix culture with our patent protected 3D Cell Matrix

Human Platelet Lysate is manufactured from platelet units obtained from healthy blood donors at either FDA-licensedor EMA licensed blood centres. The human platelets are pooled in large batch sizes (>300) to ensure outstanding consistency in availability, pricing and the product itself. The platelets are sterilized by 0.1 µm filtration, elimnating possible mycoplasma contamination. Due to high standards at all stages of of the production process, hPL is a GMP-compliant culture medium.

hPL: MycoplasmaFree
Animal Serum-Free
Filtered and tested for consistency
Approved by EMA/FDA
for Clinical Trial Usage
 hPL Pooling ensures batch-to batch consistency
hPL Pooling ensures
batch-to batch consistency

Our Products: Cell Culture Solutions based on Human Platelet Lysate

Quality Free of Animal Fibrinogen-Depleted Gamma-Irradiated For Clinical Trials
Serum Components
RUO ABIN6720610 E*
ABIN6720628 ABIN6720628 - -
GMP Quality ABIN6720613
ABIN6720608 E
ABIN6720607 E
ABIN6720624 ABIN6720613 - ABIN6720613
Clinical Quality ABIN6720621 ABIN6720632 ABIN6720632 ABIN6720621 ABIN6720621 ABIN6720632 ABIN6720632
*(Platelets are gathered from healthy blood donors at FDA-licensed blood centres in contrast to "E" marked products which are supplied by EMA-licensed blood centres )

Human Platelet Lysate enables higher Proliferation Rates

Figure 1 shows a comparison of bone marrow-derived MSC cell cultures and their respective proliferation rates. One pool of cultures used human Platelet Lysate (ABIN6720614) as cell culture media (orange), the teal data set used FBS. hPL population doubeling rate is almost twice as high in comparison to cell culture using FBS.

Population doubling

Fig 1: Cumulative population doubling of bone marrow-derived MSC was higher in human Platelet Lysate (ABIN6720614) compared to FBS.
Fig 1: Cumulative population doubling of bone marrow-derived MSC was higher in human Platelet Lysate (ABIN6720614) compared to FBS.

hPL suitable for Clinical Trials

antibodies-online offers hPL with different safety grades to match all application fields. For the highest safety level for expansion of different human cell types, gamma radiaton is applied to platelets which reduces pathogens in the final hPL to a minimum. These human platelet lysates can be used, in contrast to FCS, in therapeutical development.

Cell culture solutions based on human Platelet Lysate are suited for basic research, cellular therapeutics and clinical applications. Researchers establishing stem Cell Therapy treatments need to meet the regulatory guidelines and have a cost-effective system that is compatible with their end use. Especially for therapeutic applications, the ideal cell culture medium forgoes any animal derived components, provides equal or increased performance (e.g. cell proliferation, protein production, etc.) over serum, and is comparable in price.


Human platelets contain coagulation factors, like fibrinogen, in order to react to bleeding. Adding heparin to the medium prevents clotting reactions in the culture medium1,2. In this case the anti-coagulant (ABIN6720635) is needed to prevent clotting. hPL products which are fibrinogen-depleted don’t require additional heparin.

However addition of heparin is not advisable for every cell culture. Macrophage culture express heparan sulfate which is sufficient to act as an anticoagulant. Additionally heparin interferes with cytokine profile, releasing moreproinflammatory cytokines IL-1β -63.

>> Pharmaceutical Heparin

3D Cell Matrix

3D cell system allows to utilize a more standardized cell expansion protocol. The two-phase system consists of a human Platelet Lysate gel, which supplies your cells with nutrients and a cell culture scaffold at once. 3D Cell Culture Matrix offers a uniform distribution of growth factors throughout the cell culture system. Cells can grow in several layers at the interface layer, which minimizes contact inhibition with any artificial biomaterials. It is recommended to use Gelation Accelerator which induces prompt gelation with the 3D Cell Culture System 3D Cell Culture Matrix.

>> Gelation Accelearator
>> 3D Cell Culture Matrix

What are Cell Culture Supplements

Cell culture media supplies cells with energy and compounds which regulate the cell cycle. A typical culture medium is composed of a complement of amino acids vitamins, inorganic salts, glucose, and serum as a source of growth factors, hormones, and attachment factors. In addition to nutrients, the medium also helps maintain pH and osmolarity.

Historical usage of Fetal Calf Serum

First cell culture experiments in the early 50s used natural buffers as cell culture supplements - biological fluids and tissues extracts from living animals. Their composition is complex and hard to reconstruct, this is why natural buffers are still used predominantly today. One of the first cell culture supplements used is fetal calf serum (FCS). Fetal calf serum contains a variety of proteins, not all of which are known today. These proteins also include growth factors that are necessary for the cultivation of cells in cell culture flasks. Over the years usage of fetal calf serum, for which about one to two million animals are killed every year, is criticized more and more. Another reason to search for replacements is that cell cultures produced with FCS can hardly be registered as therapeutic cells. The large number of unknown proteins can lead to severe side effects and allergies in patients. Furthermore, calf serum can contain impurities and transmit diseases (e.g. through mycoplasma). There is now a wide range of serum-free media for the cultivation of cell lines and stem cells.

Alternatives to Fetal Calf Serum: Human Platelet Lysate

Other media such as human platelet lysate (hPL) or synthetically produced substitute sera are uncomplicated and inexpensive to produce. Since these media are produced from human material, they are better suited for the cultivation of human cells than fetal calf serum.


  1. Tylek et al. (2019): “Platelet lysate outperforms FCS and human serum for co-culture of primary human macrophages and hMSCs”, in Scientific Reports volume 9, Article number: 3533 DOI
  2. Hemeda, H et al. (2013): “Heparin concentration is critical for cell culture with human platelet lysate.”, Journal of Biological Chemistry volume15,, Article number: 1174–1181, DOI
  3. Clasper, S. et al. (1999): “Inducible Expression of the Cell Surface Heparan Sulfate Proteoglycan Syndecan-2 (Fibroglycan) on Human Activated Macrophages Can Regulate Fibroblast Growth Factor Action”, in Cytotherapy volume 274, Article number: 24113–24123, DOI

Human Platelet Lysate - GMP Quality - ABIN6720614

Superior Alternative to FBS in human Cell Culture: human Platelet Lysate
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