rCharybdotoxin

Details zu Produkt Nr. ABIN1001667, Anbieter: Anmelden zum Anzeigen
Reaktivität
Scorpion
Wirt
Escherichia coli (E. coli)
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Sequenz QFTNVSCTTS KECWSVCQRL HNTSRGKCMN KKCRCYS.
Spezifität Charybdotoxin is a potent selective inhibitor of high conductance (maxi-K), different medium and small conductance Ca2+-activated K+ channels, as well as a voltage-dependent K+ channel (Kv1.3).
Produktmerkmale Source: Leiurus quinquestriatus hebraeus (Yellow scorpion).
Molecular formula: C176H280N58O55S7.
Disulfide bonds between Cys7-Cys28, Cys13-Cys33, and Cys17-Cys35.
Reinheit > 98 %
Hintergrund Inhibition of mSlo channels heterologously expressed in HEK293 cells by Charybdotoxin. mSlo channel currents, before and during application of 100nM rCharybdotoxin. The currents were activated by a 50 ms voltage step to +100 mV from a prepulse of -180 mV with intracellular 10M Ca2+.
Molekulargewicht 4313
Applikations-hinweise 10-100 nM
Kommentare

K+ channel toxin -KTx 1.1, ChTX, ChTX-Lq1, ChTx-a.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.160ml will provide a stock solution of 10 M.
Handhabung Soluble in Water.
Bilder des Herstellers
 image for rCharybdotoxin (ABIN1001667) mSlo channel currents, before and during application of 100nM rCharybdotoxin. The cur...
Allgemeine Veröffentlichungen Meera, Wallner, Toro: "A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 10, pp. 5562-7, 2000 (PubMed).

Produkt verwendet in: Vita, Bontems, Bouet, Tauc, Poujeol, Vatanpour, Harvey, Menez, Toma: "Synthesis of charybdotoxin and of two N-terminal truncated analogues. Structural and functional characterisation." in: European journal of biochemistry / FEBS, Vol. 217, Issue 1, pp. 157-69, 1993 (PubMed).

Gimenez-Gallego, Navia, Reuben, Katz, Kaczorowski, Garcia: "Purification, sequence, and model structure of charybdotoxin, a potent selective inhibitor of calcium-activated potassium channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 10, pp. 3329-33, 1988 (PubMed).

Smith, Phillips, Miller: "Purification of charybdotoxin, a specific inhibitor of the high-conductance Ca2+-activated K+ channel." in: The Journal of biological chemistry, Vol. 261, Issue 31, pp. 14607-13, 1986 (PubMed).

Miller, Moczydlowski, Latorre, Phillips: "Charybdotoxin, a protein inhibitor of single Ca2+-activated K+ channels from mammalian skeletal muscle." in: Nature, Vol. 313, Issue 6000, pp. 316-8, 1985 (PubMed).