anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) Antikörper

The protein encoded by PPP1R14A belongs to the protein phosphatase 1 (PP1) inhibitor family. Zusätzlich bieten wir Ihnen Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A Proteine (13) und Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A Kits (1) und viele weitere Produktgruppen zu diesem Protein an.

Alle Antikörper anzeigen Gen GeneID UniProt
PPP1R14A 94274 Q96A00
PPP1R14A 114004 Q99MC0
PPP1R14A 68458 Q91VC7
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Top anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A Antikörper auf antikoerper-online.de

Showing 10 out of 61 products:

Katalog Nr. Reaktivität Wirt Konjugat Applikation Bilder Menge Anbieter Lieferzeit Preis Details
Rind (Kuh) Kaninchen Unkonjugiert WB 100 μL Anmelden zum Anzeigen 2 bis 3 Tage
$289.00
Details
Human Kaninchen Unkonjugiert IHC (p), WB Western blot analysis of CPI17 alpha using anti- CPI17 alpha antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat brain tissue lysates,   Lane 2: mouse brain tissue lysates,   Lane 3: PANC-1 whole Cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CPI17 alpha antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPI17 alpha at approximately 20KD. The expected band size for CPI17 alpha is at 20KD. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody . CPI17 alpha was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti- CPI17 alpha Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Anmelden zum Anzeigen 4 bis 6 Tage
$280.00
Details
Human Kaninchen Unkonjugiert IC, IF, IHC, WB Immunofluorescent analysis of CPI17 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibod Immunohistochemical analysis of CPI17 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the a 200 μL Anmelden zum Anzeigen 13 bis 14 Tage
$487.50
Details
Human Maus Unkonjugiert EIA, WB 0.1 mL Anmelden zum Anzeigen 6 bis 8 Tage
$412.50
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Human Kaninchen Unkonjugiert ELISA, IHC, IHC (p), WB 100 μL Anmelden zum Anzeigen 11 bis 14 Tage
$493.17
Details
Human Kaninchen Unkonjugiert ELISA, IF, IHC, IHC (p), WB 100 μL Anmelden zum Anzeigen 11 bis 14 Tage
$493.17
Details
Human Kaninchen Unkonjugiert IHC (p), WB Immunohistochemistry (IHC) analyzes of p-CPI-17 antibody in paraffin-embedded human heart tissue. Western blot (WB) analysis of p-CPI-17 antibody in extracts from RAW264.7 cell 0.1 mg Anmelden zum Anzeigen 6 bis 8 Tage
$390.50
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Human Kaninchen Unkonjugiert IF 0.1 mL Anmelden zum Anzeigen 6 bis 8 Tage
$368.50
Details
Human Kaninchen Unkonjugiert IF, IHC (p), WB Immunohistochemistry (IHC) analyzes of CPI-17 antibody in paraffin-embedded human heart tissue. Western blot (WB) analysis of CPI-17 antibody in extracts from HT-29 cells . 0.1 mg Anmelden zum Anzeigen 6 bis 8 Tage
$390.50
Details
Human Maus Unkonjugiert ELISA, WB Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (41.91 KDa) . Dilution: 1:500~1000 Western Blot analysis of PPP1R14A expression in transfected 293T cell line by PPP1R14A monoclonal antibody (M01), clone 3A7.Lane 1: PPP1R14A transfected lysate(16.7 KDa).Lane 2: Non-transfected lysate. Dilution: 1:500~1000 100 μg Anmelden zum Anzeigen 8 bis 11 Tage
$527.50
Details

Am meisten referenzierte anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A Antikörper

  1. Monoclonal PPP1R14A Primary Antibody für ELISA, WB - ABIN533777 : Lartey, Smith, Pawade, Strachan, Mellor, López Bernal: Up-regulation of myometrial RHO effector proteins (PKN1 and DIAPH1) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor. in Biology of reproduction 2007 (PubMed)
    Show all 3 Pubmed References

  2. Cow (Bovine) Polyclonal PPP1R14A Primary Antibody für WB - ABIN2789677 : Xiong, Xia, Tang, Pan, Mei, Xiong: Retromer in Osteoblasts Interacts With Protein Phosphatase 1 Regulator Subunit 14C, Terminates Parathyroid Hormone's Signaling, and Promotes Its Catabolic Response. in EBioMedicine 2016 (PubMed)

Weitere Antikörper gegen Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A Interaktionspartner

Zebrafish Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) Interaktionspartner

  1. Hoxb1b is likely to directly regulate ppp1r14al expression in rhombomere 4. Furthermore, ppp1r14al is essential for establishment of the earliest hindbrain signaling-center in rhombomere 4 by regulating expression of fgf3.

Human Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) Interaktionspartner

  1. CPI-17 drives Ras activity and tumorigenesis in melanomas in a two-fold way; inactivation of the tumor suppressor merlin and activation of the growth promoting ERM family.

  2. The authors call the mutual sequestration mechanism through which pCPI-17 and myosin light-chain phosphatase interact inhibition by unfair competition: myosin light-chain phosphatase protects pCPI-17 from other phosphatases, while pCPI-17 blocks other substrates from the active site of myosin light-chain phosphatase.

  3. Study finds that CPI-17 (PPP1R14A protein phosphatase 1 regulatory inhibitor subunit 14A, 17 kDa) is not expressed in the healthy peripheral nervous system or in nontumour pathologies of the peripheral nervous system. CPI-17 up-regulation, however, is observed in over 90% of schwannomas, but not in neurofibroma and only rarely in malignant peripheral nerve sheath tumour.

  4. CPI-17 has a role in restoring skin homoeostasis in cutaneous field of cancerization lesions

  5. analysis of the interaction between plakoglobin and CPI-17, which is affected by the phosphorylation status of CPI-17 in human lung microvascular endothelial cells

  6. The N-terminal 21-residue tail domain of CPI-17 is necessary for nuclear localization. Phospho-mimetic Asp-substitution of CPI-17 at Ser12 attenuates the nuclear import.

  7. The study characterized the CPI-17 promoter and identified binding sites for GATA-6 and nuclear factor kappa B (NF-kappaB).

  8. a novel signaling cascade that links RhoA-mediated calcium sensitivity to MEF2-dependent myocardin expression in VSMCs through a mechanism involving p38 MAPK, PP1alpha, and CPI-17.

  9. RACK1 may play a role in PKC/CPI-17 signaling pathway.

  10. cAMP/PKA regulates the endothelial barrier via inhibition of the contractile machinery, mainly by the activation of MLCP via inhibition of CPI-17 and RhoA/Rock.

  11. mapped to chromosome 19q13.13-q13.2

  12. CPI-17 binds directly to protein kinase C and casein kinase I.

  13. importance of PKC/CPI-17-mediated pathway in histamine-triggered cytoskeletal rearrangements leading to lung microvascular barrier compromise.

  14. CPI-17 siRNA decreased the level of merlin phosphorylation and consequently Ras and ERK activity in human tumor cell lines

  15. These data indicate that 14,15-epoxyeicosatrienoic acid hyperpolarizes airway smooth muscle cells and relaxes precontracted human bronchi and intracellular effects are related to a PKC-dependent process involving a lower phosphorylation level of CPI-17.

  16. Human pregnancy is characterized by increases in PKN1 expression and PPP1R14A phosphorylation in the myometrium.

  17. These results are consistent with a key molecular role for CPI-17 in airway hyperresponsiveness, in the absence of bronchial wall remodeling.

  18. NF2 is rendered inactive by phosphorylation of Ser 518 and this can be explained at least in part by an increased expression of CPI-17.

Mouse (Murine) Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) Interaktionspartner

  1. CPI-17 phosphorylation at T38 is essential for the maintenance of physiological blood pressure.

  2. Enhanced vasorelaxation in early endotoxemia is mediated by redox signaling through PKG-1alpha but in later endotoxemia by myosin phosphatase isoform shifts enhancing sensitivity to NO/cGMP as well as smooth muscle atrophy.

  3. The study characterized the CPI-17 promoter and identified binding sites for GATA-6 and nuclear factor kappa B (NF-kappaB).

  4. a novel signaling cascade that links RhoA-mediated calcium sensitivity to MEF2-dependent myocardin expression in VSMCs through a mechanism involving p38 MAPK, PP1alpha, and CPI-17.

  5. Data suggest that CPI-17 and both conventional and novel PKC isozymes contribute to the phasic and tonic contractile components of BALB/c colonic circular smooth muscle.

  6. Organ-specific mechanisms involving MYPT1, M-RIP, and CPI-17 are critical to regulating basal LC20 phosphorylation in gastrointestinal smooth muscles.

  7. calcium-independent phospholipase A2beta has a role in high glucose-induced activation of RhoA, Rho kinase, and CPI-17 in cultured vascular smooth muscle cells and vascular smooth muscle hypercontractility in diabetic animals

  8. distribution of mouse and rat PPP1R14A was more specific and different from that of humans

  9. concluded that actions downstream of cGMP/PKG can reverse PKC-mediated phosphorylation of CPI-17 and Ca(2+) sensitization in smooth muscle

  10. findings suggest that CPI-17 was downregulated during intestinal inflammation and that TNF-alpha plays a central role in this process. Downregulation of CPI-17 may play a role in motility impairments in inflammation.

  11. CPI-17 expression is reversibly controlled in response to the phenotype transition of smooth muscle cells that restricts the signal to differentiated smooth muscle cells and particular cell types.

Pig (Porcine) Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) Interaktionspartner

  1. This study examined the protein expression and spatial-temporal distribution of PKCalpha and CPI-17 in intact smooth muscle tissues.

Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) Antigen-Profil

Protein Überblick

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Genbezeichner und Symbole assoziert mit PPP1R14A

  • protein phosphatase 1 regulatory inhibitor subunit 14A L homeolog (ppp1r14a.L) Antikörper
  • protein phosphatase 1 regulatory inhibitor subunit 14A (ppp1r14a) Antikörper
  • protein phosphatase 1, regulatory (inhibitor) subunit 14Aa (ppp1r14aa) Antikörper
  • protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A) Antikörper
  • protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A) Antikörper
  • protein phosphatase 1, regulatory (inhibitor) subunit 14A (Ppp1r14a) Antikörper
  • 1110001M11Rik Antikörper
  • CPI-17 Antikörper
  • Cpi17 Antikörper
  • im:7147597 Antikörper
  • MGC81874 Antikörper
  • PPP1INL Antikörper
  • PPP1R14A Antikörper
  • zgc:110700 Antikörper

Bezeichner auf Proteinebene für PPP1R14A

protein phosphatase 1, regulatory (inhibitor) subunit 14A , protein phosphatase 1 regulatory subunit 14A , 17 kDa PKC-potentiated inhibitory protein of PP1 , 17-KDa protein , 17-kDa PKC-potentiated inhibitory protein of PP1 , PKC-potentiated inhibitory protein of PP1 , protein kinase C-potentiated inhibitor protein of 17 kDa , CPI-17

GENE ID SPEZIES
447728 Xenopus laevis
493331 Xenopus (Silurana) tropicalis
550561 Danio rerio
612579 Canis lupus familiaris
615348 Bos taurus
641304 Ovis aries
747250 Pan troglodytes
94274 Homo sapiens
114004 Rattus norvegicus
68458 Mus musculus
397610 Sus scrofa
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