anti-Schwein WNT4 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT4 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Wingless-Type MMTV Integration Site Family, Member 4 (WNT4) Antikörper
  • CG4698
  • DWnt-4
  • DWnt4
  • Dm DWnt4
  • Dmel\\CG4698
  • Dwnt4
  • Wnt
  • Wnt-4
  • anon-EST:Liang-2.4
  • clone 2.4
  • wnt-4
  • wnt4
  • SERKAL
  • WNT-4
  • Xwnt4
  • xwnt-4
  • zgc:136737
  • Wnt family member 4
  • Wnt oncogene analog 4
  • wingless-type MMTV integration site family, member 4
  • wingless-type MMTV integration site family member 4 S homeolog
  • wingless-type MMTV integration site family, member 4a
  • WNT4
  • Wnt4
  • wnt4.S
  • wnt4a
Epitop
Internal Region
29
18
15
12
6
6
5
3
2
2
2
2
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus)
117
70
46
4
4
4
4
3
3
3
3
2
1
1
Wirt
Kaninchen
94
18
18
5
Klonalität
Polyklonal
Konjugat
Dieser WNT4 Antikörper ist unkonjugiert
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
104
35
30
30
13
6
4
2
2
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1049477
$ 451.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1049478 IHC (p) Rabbit Internal Region Anmelden zum Anzeigen Polyclonal

General

Antigen Wingless-Type MMTV Integration Site Family, Member 4 (WNT4) Antikörper
Epitop Internal Region
(29), (18), (15), (12), (6), (6), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus)
(117), (70), (46), (4), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1)
Wirt Kaninchen
(94), (18), (18), (5)
Klonalität
Polyklonal
Konjugat Dieser WNT4 Antikörper ist unkonjugiert
(4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(104), (35), (30), (30), (13), (6), (4), (2), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-WNT4 Antikörper

Target Details WNT4 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human WNT4. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except WNT5A (71 % ).
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (100%) Opossum (86%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 14 amino acid peptide from internal region of human WNT4. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (100%), Opossum (86%).

Type of Immunogen: Synthetic peptide

Target Details WNT4

Produktdetails anti-WNT4 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung WNT4 (WNT4 Antibody Abstract)
Hintergrund Name/Gene ID: WNT4
Family: WNT

Synonyms: WNT4, WNT-4, Protein Wnt-4, SERKAL
Gen-ID 54361
Forschungsgebiet Organogenesis, Reproduction, Wnt
Pathways WNT Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cell-Cell Junction Organization, Tube Formation

Anwendungsinformationen

Produktdetails anti-WNT4 Antikörper Target Details WNT4 Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (10 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-WNT4 Antikörper Target Details WNT4 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-WNT4 Antikörper Target Details WNT4 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 4 (WNT4) (Internal Region) antibody (ABIN1049477) Anti-WNT4 antibody ABIN1049477 IHC staining of human thyroid. Immunohistochemistry o...