anti-Ratte (Rattus) WNT2 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Wingless-Type MMTV Integration Site Family Member 2 (WNT2) Antikörper
  • CG1916
  • D-wnt-2
  • DWnt-2
  • DWnt2
  • Dm DWnt2
  • Dm-2
  • Dmel\\CG1916
  • Dwnt-2
  • Wnt
  • Wnt-2
  • dWnt2
  • wnt2
  • WNT2
  • irp
  • Xwnt2
  • wnt-2
  • Xwnt-2
  • int1l1
  • 2610510E18Rik
  • Int1l1
  • Irp
  • Mirp
  • Wnt2a
  • INT1L1
  • IRP
  • ZfWnt2
  • Wnt oncogene analog 2
  • wingless-type MMTV integration site family member 2
  • wingless-related MMTV integration site 2
  • Wnt2
  • wnt2
  • LOC100221216
  • WNT2
Epitop
AA 240-290
14
6
3
3
2
2
2
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
65
38
31
18
4
4
4
4
4
4
3
2
2
Wirt
Kaninchen
67
Klonalität
Polyklonal
Konjugat
Dieser WNT2 Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
41
14
12
10
5
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN762896
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN762905 IHC (p) WB HRP Rabbit IgG AA 240-290 Anmelden zum Anzeigen Polyclonal
1 ABIN762898 IHC (p) WB Biotin Rabbit IgG AA 240-290 Anmelden zum Anzeigen Polyclonal

General

Antigen Wingless-Type MMTV Integration Site Family Member 2 (WNT2) Antikörper
Epitop AA 240-290
(14), (6), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reaktivität Hund, Human, Maus, Ratte (Rattus)
(65), (38), (31), (18), (4), (4), (4), (4), (4), (4), (3), (2), (2)
Wirt Kaninchen
(67)
Klonalität
Polyklonal
Konjugat Dieser WNT2 Antikörper ist unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(41), (14), (12), (10), (5), (1), (1), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-WNT2 Antikörper

Target Details WNT2 Anwendungsinformationen Handhabung Referenzen für anti-WNT2 Antikörper (ABIN762896) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human WNT2
Isotyp IgG

Target Details WNT2

Produktdetails anti-WNT2 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-WNT2 Antikörper (ABIN762896) Bilder zurück nach oben
Antigen
Andere Bezeichnung WNT2 (WNT2 Antibody Abstract)
Hintergrund

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

Subcellular location: Secreted

Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2

Gen-ID 7472
UniProt P09544
Forschungsgebiet Cancer, Wnt
Pathways WNT Signalweg

Anwendungsinformationen

Produktdetails anti-WNT2 Antikörper Target Details WNT2 Handhabung Referenzen für anti-WNT2 Antikörper (ABIN762896) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-WNT2 Antikörper Target Details WNT2 Anwendungsinformationen Referenzen für anti-WNT2 Antikörper (ABIN762896) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-WNT2 Antikörper (ABIN762896)

Produktdetails anti-WNT2 Antikörper Target Details WNT2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, 2014

Bilder

Produktdetails anti-WNT2 Antikörper Target Details WNT2 Anwendungsinformationen Handhabung Referenzen für anti-WNT2 Antikörper (ABIN762896) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Formalin-fixed and paraffin embedded human placenta labeled with Anti-WNT2 Polyclonal...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Images provided the Independent Validation Program (badge number 029629)Formalin-fixe...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Images provided the Independent Validation Program (badge number 029629)Formalin-fixe...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown i...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded ca...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Mouse brain lysates probed with Anti-WNT2 Polyclonal Antibody, Unconjugated at 1:500...
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Image provided by the Independent Validation Program (badge number 29808). Lane 1: A5...
'Independent Validation' Siegel
Chargennummer 131105
Validierte Anwendung Immunohistochemistry
Positivkontrolle Human glioma tissue
Negativkontrolle Human brain
Bewertung Signal was detected in positive control tissue and not in negative control tissue.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Human Glioma (specimen known to contain the target protein) from HTSR’s Human Tissue Bank.
  • Negative Control: Human Normal Brain (specimen known to not contain the target protein) from HTSR’s Human Tissue Bank.
  • Primary antibody isotype control: Human Glioma treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Human Glioma treated with secondary antibody only (no primary antibody).
Protokoll
  • Immunohistochemistry was performed by hand.
  • Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
  • Sections were rinsed in de-ionized water for 5 min at RT.
  • Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
  • Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
  • Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
  • Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
  • Sections were rinsed in TBST for 5 min 2X at RT.
  • Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
  • Sections were rinsed in TBST for 5 min 2X at RT.
  • Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
  • Sections were washed x 1 in Distilled Water.
  • Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were blued in Ammonium Hydroxide for 1 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
Anmerkungen
  • The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
  • The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
Validierungsbilder
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 1: Human brain glioma tissue stained with anti-Wnt2 (brown) and counterstained...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 2: Human normal brain tissue stained with anti-Wnt2 (brown) and counterstained...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 3: Human brain glioma tissue stained with isotype control antibody (brown) and...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 4: Human brain glioma tissue stained with secondary only (brown) and counterst...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 240-290) antibody (ABIN762896) Figure 5: Human brain tissue with Parkinson's morphology stained with anti-Wnt2 (brow...