anti-Kaninchen WNT1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antikörper
  • Xint-1
  • Xwnt1
  • int-1
  • int1
  • wnt-1
  • wnt1-a
  • Int-1
  • Wnt-1
  • sw
  • swaying
  • BMND16
  • INT1
  • OI15
  • Int1
  • WNT-1
  • sb:eu647
  • zgc:194464
  • zgc:194478
  • WNT1
  • Wnt family member 1
  • Wnt family member 1 L homeolog
  • wingless-type MMTV integration site family, member 1
  • WNT1
  • wnt1.L
  • Wnt1
  • wnt1
Epitop
Internal Region
15
14
8
6
2
2
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Pferd, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
114
51
22
11
8
7
6
5
5
4
3
3
2
2
1
1
Wirt
Kaninchen
94
20
Klonalität
Polyklonal
Konjugat
Dieser WNT1 Antikörper ist unkonjugiert
5
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
92
48
33
31
19
17
7
6
2
2
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1049465
$ 451.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1049466 IHC (p) Rabbit Internal Region Anmelden zum Anzeigen Polyclonal

General

Antigen Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antikörper
Epitop Internal Region
(15), (14), (8), (6), (2), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Pferd, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
(114), (51), (22), (11), (8), (7), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1)
Wirt Kaninchen
(94), (20)
Klonalität
Polyklonal
Konjugat Dieser WNT1 Antikörper ist unkonjugiert
(5), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(92), (48), (33), (31), (19), (17), (7), (6), (2), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-WNT1 Antikörper

Target Details WNT1 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human WNT1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (100%) Marmoset, Hamster, Chicken, Lizard (95%) Stickleback (84%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 19 amino acid peptide from internal region of human WNT1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (100%), Marmoset, Hamster, Chicken, Lizard (95%), Stickleback (84%).

Type of Immunogen: Synthetic peptide

Target Details WNT1

Produktdetails anti-WNT1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung WNT1 (WNT1 Antibody Abstract)
Hintergrund Name/Gene ID: WNT1
Family: WNT

Synonyms: WNT1, INT1, Proto-oncogene Int-1 homolog, Proto-oncogene Wnt-1
Gen-ID 7471
Pathways WNT Signalweg, Dopaminergic Neurogenesis

Anwendungsinformationen

Produktdetails anti-WNT1 Antikörper Target Details WNT1 Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (10 - 15 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
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Handhabung

Produktdetails anti-WNT1 Antikörper Target Details WNT1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-WNT1 Antikörper Target Details WNT1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Internal Region) antibody (ABIN1049465) Anti-WNT1 antibody ABIN1049465 IHC staining of human kidney. Immunohistochemistry of...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Internal Region) antibody (ABIN1049465) Anti-WNT1 antibody ABIN1049465 IHC staining of human small intestine. Immunohistoche...