anti-Maus DVL1 Antikörper für Immunofluorescence (Paraffin-embedded Sections)

Recommended DVL1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) Antikörper
  • Xdsh
  • dsh1
  • dvl-1
  • DVL
  • DVL1L1
  • DVL1P1
  • DSH
  • DVL-22
  • DVL1
  • DVL4
  • Dvl
  • mKIAA4029
  • DVL-1
  • dvl1
  • dvl2l
  • dishevelled segment polarity protein 1
  • dishevelled segment polarity protein 1 pseudogene 1
  • dishevelled, dsh homolog 1 (Drosophila)
  • dishevelled, dsh homolog 1b (Drosophila)
  • dvl1
  • Dvl1
  • DVL1
  • DVL1P1
  • dvl1b
Epitop
AA 20-70
14
14
10
10
9
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
61
44
28
17
9
5
4
3
3
2
2
2
1
1
1
Wirt
Kaninchen
61
23
3
Klonalität
Polyklonal
Konjugat
Dieser DVL1 Antikörper ist unkonjugiert
7
7
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
74
49
22
16
12
8
8
3
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN670671
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN670677 IF (p) Cy7 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN670678 IF (p) FITC Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN670675 IF (p) Cy5 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN670676 IF (p) Cy5.5 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN670674 IF (p) Cy3 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN894624 IF (p) Alexa Fluor 647 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN894625 IF (p) Alexa Fluor 350 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN894626 IF (p) Alexa Fluor 488 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN894627 IF (p) Alexa Fluor 555 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN2812561 IF (p) Alexa Fluor 594 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN5001573 IF (p) Alexa Fluor 680 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal
1 ABIN5001574 IF (p) Alexa Fluor 750 Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal

General

Antigen Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) Antikörper
Epitop AA 20-70
(14), (14), (10), (10), (9), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(61), (44), (28), (17), (9), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(61), (23), (3)
Klonalität
Polyklonal
Konjugat Dieser DVL1 Antikörper ist unkonjugiert
(7), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(74), (49), (22), (16), (12), (8), (8), (3), (2), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-DVL1 Antikörper

Target Details DVL1 Anwendungsinformationen Handhabung Referenzen für anti-DVL1 Antikörper (ABIN670671) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human DVL1
Isotyp IgG

Target Details DVL1

Produktdetails anti-DVL1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-DVL1 Antikörper (ABIN670671) Bilder zurück nach oben
Antigen
Andere Bezeichnung DVL1 (DVL1 Antibody Abstract)
Hintergrund

Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).

Subcellular location: Cytoplasm, Cell membrane

Synonyms: DVL, DVL1L1, DVL1P1, Segment polarity protein dishevelled homolog DVL-1, Dishevelled-1, DSH homolog 1, DVL1

Gen-ID 1855
UniProt O14640
Pathways WNT Signalweg, Synaptic Membrane, Skeletal Muscle Fiber Development

Anwendungsinformationen

Produktdetails anti-DVL1 Antikörper Target Details DVL1 Handhabung Referenzen für anti-DVL1 Antikörper (ABIN670671) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-DVL1 Antikörper Target Details DVL1 Anwendungsinformationen Referenzen für anti-DVL1 Antikörper (ABIN670671) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-DVL1 Antikörper (ABIN670671)

Produktdetails anti-DVL1 Antikörper Target Details DVL1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Hua, Xu, He, Jiang, Ye, Pan: "Wnt4/?-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8421-31, 2015

Bilder

Produktdetails anti-DVL1 Antikörper Target Details DVL1 Anwendungsinformationen Handhabung Referenzen für anti-DVL1 Antikörper (ABIN670671) zurück nach oben
Bilder des Herstellers
SDS-PAGE (SDS) image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Lane 1: rat brain lysates Lane 2: rat lung lysates probed with Anti DVL1/Dishevelled ...
Immunohistochemistry (IHC) image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Images provided the Independent Validation Program (badge number 029657)Formalin-fixe...
'Independent Validation' Siegel
Chargennummer 140106
Validierte Anwendung Immunohistochemistry
Positivkontrolle Human kidney tubules
Negativkontrolle Human colon stromal tissue
Bewertung While faint, signal is detectable in positive control tissue and not in negative control tissue. Note that staining is expected in colon epithelial cells, and is not expected in kidney glomeruli.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Human kidney tissue stained with antibody
  • Negative control: Human colon tissue stained with antibody
  • Isotype control: Human kidney tissue stained with isotype control
  • Secondary only control: Human kidney tissue stained with secondary antibody only
Protokoll
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Anmerkungen
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validierungsbilder
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 1: Human kidney tissue stained with anti-DVL1 (brown) and counterstained with ...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 2: Human colon tissue stained with anti-DVL1 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 3: Human kidney tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 4: Human kidney tissue stained with secondary only (brown) and counterstained ...