A549 Cell Slide

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Wirt
Human
Applikation
Immunocytochemistry (ICC)
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Protein Source Cells
Produktmerkmale Human A549 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The A549 cells were fixed in 4 % paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wells surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 A549 cells. A follow-up heat dehydration process was done to ensure the attachment of A549 cells and the stability of cellular proteins.
Fixation: 4 % paraformaldehyde
Tissue or Cell type A549
Bestandteile A549 Cell Slide
Lysate Type Cell Line
Lysed Cells A549 Cells
Applikationshinweise A549 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1 % Triton X-100 pretreatment for 1 minute at room temperature is highly suggested.
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Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C
Informationen zur Lagerung A549 cell slide can be stored for up to 6 months at 4 °C under desiccate conditions.
Haltbarkeit 6 months