HeLa Cell Slide

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Wirt
Human
Applikation
Immunocytochemistry (ICC)
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Protein Source Cells
Produktmerkmale Human HeLa S3 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The Hela cells were fixed in 4 % paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wells surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 Hela cells. A follow-up heat dehydration process was done to ensure the attachment of Helacells and the stability of cellular proteins.
Fixation: 4 % paraformaldehyde
Tissue or Cell type HeLa
Bestandteile HeLa Cell Slide
Lysate Type Cell Line
Lysed Cells HeLa Cells
Applikationshinweise Hela cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1 % Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.
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Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C
Informationen zur Lagerung Hela cell slides can be stored for up to 6 months at 4 °C under desiccate conditions.
Haltbarkeit 6 months