anti-Maus CD3E Antikörper für Cytotoxicity Test

Recommended CD3E Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antikörper
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reaktivität
Maus
482
460
121
87
53
34
29
29
28
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Wirt
Armenischer Hamster
437
225
188
58
21
15
3
1
Klonalität
Monoklonal
Konjugat
Dieser CD3E Antikörper ist unkonjugiert
89
75
73
32
24
22
21
20
20
18
17
16
14
14
14
14
14
12
12
12
12
10
8
8
7
7
6
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
Applikation
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
696
324
226
200
143
139
111
96
94
42
29
18
13
9
8
7
6
4
3
3
3
2
2
2
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN967568
79,99 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN967370 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Anmelden zum Anzeigen 145-2C11 13
1 ABIN1176869 BR CyTox FM IHC (fro) FACS IP WB Armenian Hamster IgG1, kappa Anmelden zum Anzeigen 145-2C11 10

anti-CD3E Antikörper für Cytotoxicity Test mit den meisten Publikationen

Ähnliche anti-CD3E Antikörper

Applikation / Reaktivität Maus
Activity Assay (AcA) 1 Antikörper
Blocking Reagent (BR) 6 Antikörper
Cytometry by Time of Flight (CyTOF) 12 Antikörper
Cytotoxicity Test (CyTox) 3 Antikörper
Depletion (Dep) 3 Antikörper
ELISA 16 Antikörper
ELISA (Capture) 6 Antikörper
ELISpot (Capture) (ELISPOT (Capture)) 2 Antikörper
Flow Cytometry (FACS) 385 Antikörper
Fluorescence Microscopy (FM) 1 Antikörper
Functional Studies (Func) 11 Antikörper
Immunocytochemistry (ICC) 32 Antikörper
Immunofluorescence (IF) 56 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 7 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antikörper
Immunohistochemistry (IHC) 105 Antikörper
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 40 Antikörper

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antikörper
Reaktivität Maus
(482), (460), (121), (87), (53), (34), (29), (29), (28), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Wirt Armenischer Hamster
(437), (225), (188), (58), (21), (15), (3), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser CD3E Antikörper ist unkonjugiert
(89), (75), (73), (32), (24), (22), (21), (20), (20), (18), (17), (16), (14), (14), (14), (14), (14), (12), (12), (12), (12), (10), (8), (8), (7), (7), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Applikation Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(696), (324), (226), (200), (143), (139), (111), (96), (94), (42), (29), (18), (13), (9), (8), (7), (6), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 13 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CD3E Antikörper

Target Details CD3E Anwendungsinformationen Handhabung ProductDetails: References for anti-CD3E antibody (ABIN967568) Bilder
Marke BD Pharmingen™
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen H-2Kb specific cytotoxic T lymphocyte clone BM10-37
Klon 145-2C11
Isotyp IgG1, kappa

Target Details CD3E

Produktdetails anti-CD3E Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-CD3E antibody (ABIN967568) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD3e (CD3E Antibody Abstract)
Hintergrund The 145-2C11 antibody reacts with the 25-kDa epsilon chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3epsilon and anti-alphabeta TCR mAbs to detect the T-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis.
Synonyms: CD3epsilon chain
Forschungsgebiet CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-CD3E Antikörper Target Details CD3E Handhabung ProductDetails: References for anti-CD3E antibody (ABIN967568) Bilder zurück nach oben
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CD3E Antikörper Target Details CD3E Anwendungsinformationen ProductDetails: References for anti-CD3E antibody (ABIN967568) Bilder zurück nach oben
Format Liquid
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4° C.

ProductDetails: References for anti-CD3E antibody (ABIN967568)

Produktdetails anti-CD3E Antikörper Target Details CD3E Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Martin, Lenardo: "Morphological, biochemical, and flow cytometric assays of apoptosis." in: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Vol. Chapter 14, pp. Unit 14.13, 2008

Kruisbeek, Shevach, Thornton: "Proliferative assays for T cell function." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 3, pp. Unit 3.12, 2008

Chai, Lechler: "Immobilized anti-CD3 mAb induces anergy in murine naive and memory CD4+ T cells in vitro." in: International immunology, Vol. 9, Issue 7, pp. 935-44, 1997

Nakano, Yamazaki, Miyatake, Nozaki, Kikuchi, Saito: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, 1996

Wagner, Hagman, Linsley, Hodsdon, Freed, Newell: "Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1631-8, 1996

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994

Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." in: International immunology, Vol. 6, Issue 7, pp. 995-1001, 1994

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, 1989

Portoles, Rojo, Golby, Bonneville, Gromkowski, Greenbaum, Janeway, Murphy, Bottomly: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, 1989

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987

Allgemeine Veröffentlichungen

Isakov, Wange, Burgess, Watts, Aebersold, Samelson: "ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity." in: The Journal of experimental medicine, Vol. 181, Issue 1, pp. 375-80, 1995

Bilder

Produktdetails anti-CD3E Antikörper Target Details CD3E Anwendungsinformationen Handhabung ProductDetails: References for anti-CD3E antibody (ABIN967568) zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD3E Antikörper (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967568) CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained ...
 image for anti-CD3E Antikörper (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967568) BALB/c thymocytes were also stained with purified anti-mouse CD3e 145-2C11 or isotype...
 image for anti-CD3E Antikörper (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967568) Purified anti-mouse CD3e mAb 145-2C11