anti-Maus VASP Antikörper für Enzyme Immunoassay

Recommended VASP Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Vasodilator-Stimulated phosphoprotein (VASP) Antikörper
  • vasp
  • MGC80889
  • wu:fb23b04
  • wu:fk84g05
  • zgc:110347
  • si:dkey-113g17.1
  • si:ch211-202c21.1
  • DDBDRAFT_0188463
  • DDBDRAFT_0229340
  • DDB_0188463
  • DDB_0229340
  • VASP
  • vasodilator-stimulated phosphoprotein
  • Vasodilator-stimulated phosphoprotein
  • zgc:162320
  • vasp
  • AaeL_AAEL010747
  • CpipJ_CPIJ004706
  • CpipJ_CPIJ004707
  • vasP
  • VASP
  • zgc:162320
  • Vasp
Epitop
pSer157
51
24
19
15
12
8
7
6
6
4
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
275
145
116
20
16
13
8
7
5
3
2
1
1
Wirt
Maus
226
41
8
Klonalität
Monoklonal
Konjugat
Dieser VASP Antikörper ist unkonjugiert
7
6
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
246
119
67
50
48
33
26
18
15
11
4
4
2
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN126908
$ 610.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN126905 EIA FACS IF IP WB Biotin Mouse IgG1 pSer157 Anmelden zum Anzeigen 5C6 1
1 ABIN126913 EIA FACS WB Mouse IgG1 pSer239 Anmelden zum Anzeigen 2-20E-012
1 ABIN126909 EIA FACS IF IP WB Biotin Mouse IgG1 pSer239 Anmelden zum Anzeigen 16C2
1 ABIN126912 EIA FACS IF IP WB Mouse IgG1 pSer239 Anmelden zum Anzeigen 16C2

General

Antigen Vasodilator-Stimulated phosphoprotein (VASP) Antikörper
Epitop pSer157
(51), (24), (19), (15), (12), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(275), (145), (116), (20), (16), (13), (8), (7), (5), (3), (2), (1), (1)
Wirt Maus
(226), (41), (8)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser VASP Antikörper ist unkonjugiert
(7), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(246), (119), (67), (50), (48), (33), (26), (18), (15), (11), (4), (4), (2), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-VASP Antikörper

Target Details VASP Anwendungsinformationen Handhabung Referenzen für anti-VASP Antikörper (ABIN126908) Bilder
Spezifität AM00155PU-N recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not cross-react with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, AM00155PU-N (clone 5C6) is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.
Produktmerkmale Synonyms: Vasodilator-stimulated phosphoprotein
Reinigung Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Immunogen Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 157 RRVpSNAG
Klon 5C6
Isotyp IgG1

Target Details VASP

Produktdetails anti-VASP Antikörper Anwendungsinformationen Handhabung Referenzen für anti-VASP Antikörper (ABIN126908) Bilder zurück nach oben
Antigen
Andere Bezeichnung VASP (VASP Antibody Abstract)
Hintergrund VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Molecular Weight: 46/50 kDaSynonyms: Vasodilator-stimulated phosphoprotein
Gen-ID 7408
UniProt P50552
Forschungsgebiet Signaling, Extracellular Matrix
Pathways T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation

Anwendungsinformationen

Produktdetails anti-VASP Antikörper Target Details VASP Handhabung Referenzen für anti-VASP Antikörper (ABIN126908) Bilder zurück nach oben
Applikationshinweise Western blot ( 0.5 μg/mL for HRPO/ECL detection). Recommended: Casein/Tween 20 basedblocking and blot incubation buffer. ELISA (0.05 μg/mL). Immunoprecipitation (1 - 10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1 - 10 μg/mL, may tolerate 0.5 % formaldehyde fixation). Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-VASP Antikörper Target Details VASP Anwendungsinformationen Referenzen für anti-VASP Antikörper (ABIN126908) Bilder zurück nach oben
Rekonstitution Restore with 1 mL H2O (15 min, RT).
Buffer PBS with 0.09 % Sodium Azide, PEG and Sucrose
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!
Haltbarkeit 12 months

Referenzen für anti-VASP Antikörper (ABIN126908)

Produktdetails anti-VASP Antikörper Target Details VASP Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Iyú, Glenn, White, Fox, Heptinstall: "Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, 2011

Bilder

Produktdetails anti-VASP Antikörper Target Details VASP Anwendungsinformationen Handhabung Referenzen für anti-VASP Antikörper (ABIN126908) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody (ABIN126908) Phosphospecificity: Whole cell extracts of control (co) or SNP treat...